IL-7 production in murine lymphatic endothelial cells and induction in the setting of peripherallymphopenia

Corey N. Miller, Dennis Hartigan-O'Connor, Myeong Sup Lee, Grace Laidlaw, Ivo P. Cornelissen, Mehrdad Matloubian, Shaun R. Coughlin, Donald M. McDonald, Joseph M. McCune

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

IL-7 is a required factor for T-cell homeostasis. Because of low expression levels and poor reagent availability, the cellular sources of IL-7 have proven challenging to characterize. In this study, we describe a reporter mouse in which enhanced GFP is expressed from the endogenous Il7 locus. We show that IL-7 is produced by lymphatic endothelial cells (LECs) distributed throughout the systemic lymphatic vasculature as well as by fibroblastic reticular cells, and that phosphorylation of STAT5 in lymphocytes is higher in lymphatics than in blood. Furthermore, in nodes depleted of lymphocytes, Il7 transcription is increased in stromal but not in myeloid subsets. These data support recent findings that lymphocyte homeostasis is influenced by access to secondary lymphoid organs and point to LECs as an important in vivo source of IL-7, bathing trafficking immune cells under both resting and lymphopenic conditions.

Original languageEnglish (US)
Pages (from-to)471-483
Number of pages13
JournalInternational Immunology
Volume25
Issue number8
DOIs
StatePublished - Aug 2013

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Keywords

  • Endothelium
  • HIV
  • Interleukin-7
  • Lymph node
  • Lymphatic
  • Lymphopenia
  • Myeloid
  • Stromal

ASJC Scopus subject areas

  • Immunology

Cite this

Miller, C. N., Hartigan-O'Connor, D., Lee, M. S., Laidlaw, G., Cornelissen, I. P., Matloubian, M., Coughlin, S. R., McDonald, D. M., & McCune, J. M. (2013). IL-7 production in murine lymphatic endothelial cells and induction in the setting of peripherallymphopenia. International Immunology, 25(8), 471-483. https://doi.org/10.1093/intimm/dxt012