IL-2 modulation of murine T-cell oncogene expression (42465)

Michael F Seldin, J. D. Mountz, J. F. Mushinski, H. R. Smith, A. D. Steinberg

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

C-myb, a cellular oncogene associated with normal thymic development, was found to be highly expressed in four interleukin 2 (IL-2)-independent T-cell lines, but not in two of three IL-2-dependent T-cell lines. The IL-2-dependent lines, HT2 and CTLL-2, were found to have low levels of c-myb mRNA in the presence of IL-2. However, short-term IL-2 depletion resulted in at least fivefold increases in c-myb message. Add-back of IL-2 after 30 hr IL-2-depletion of CTLL-2 cells resulted in return to baseline low-level c-myb mRNA. Expression of the oncogenes myc, bas, raf, and abl as well as the T-cell genes Thy-1 and C(T)β did not parallel that of c-myb. These studies indicate that removal of a growth factor can result in increased levels of a specific cellular oncogene and that two nuclear protooncogenes (c-myb and c-myc) are expressed differentially during cell growth. These results may help to explain aspects of intrathymic T-cell differentiation where there is very high c-myb expression in the fact of limiting amounts of growth factors such as IL-2.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume184
Issue number2
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Seldin, M. F., Mountz, J. D., Mushinski, J. F., Smith, H. R., & Steinberg, A. D. (1987). IL-2 modulation of murine T-cell oncogene expression (42465). Proceedings of the Society for Experimental Biology and Medicine, 184(2), 186-190.