IL-2 modulation of murine T-cell oncogene expression (42465)

Michael F Seldin, J. D. Mountz, J. F. Mushinski, H. R. Smith, A. D. Steinberg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

C-myb, a cellular oncogene associated with normal thymic development, was found to be highly expressed in four interleukin 2 (IL-2)-independent T-cell lines, but not in two of three IL-2-dependent T-cell lines. The IL-2-dependent lines, HT2 and CTLL-2, were found to have low levels of c-myb mRNA in the presence of IL-2. However, short-term IL-2 depletion resulted in at least fivefold increases in c-myb message. Add-back of IL-2 after 30 hr IL-2-depletion of CTLL-2 cells resulted in return to baseline low-level c-myb mRNA. Expression of the oncogenes myc, bas, raf, and abl as well as the T-cell genes Thy-1 and C(T)β did not parallel that of c-myb. These studies indicate that removal of a growth factor can result in increased levels of a specific cellular oncogene and that two nuclear protooncogenes (c-myb and c-myc) are expressed differentially during cell growth. These results may help to explain aspects of intrathymic T-cell differentiation where there is very high c-myb expression in the fact of limiting amounts of growth factors such as IL-2.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume184
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

T-cells
Oncogenes
Interleukin-2
Modulation
T-Lymphocytes
Intercellular Signaling Peptides and Proteins
Cell Line
Messenger RNA
myc Genes
Cell growth
Cell Differentiation
Genes
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Seldin, M. F., Mountz, J. D., Mushinski, J. F., Smith, H. R., & Steinberg, A. D. (1987). IL-2 modulation of murine T-cell oncogene expression (42465). Proceedings of the Society for Experimental Biology and Medicine, 184(2), 186-190.

IL-2 modulation of murine T-cell oncogene expression (42465). / Seldin, Michael F; Mountz, J. D.; Mushinski, J. F.; Smith, H. R.; Steinberg, A. D.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 184, No. 2, 1987, p. 186-190.

Research output: Contribution to journalArticle

Seldin, MF, Mountz, JD, Mushinski, JF, Smith, HR & Steinberg, AD 1987, 'IL-2 modulation of murine T-cell oncogene expression (42465)', Proceedings of the Society for Experimental Biology and Medicine, vol. 184, no. 2, pp. 186-190.
Seldin, Michael F ; Mountz, J. D. ; Mushinski, J. F. ; Smith, H. R. ; Steinberg, A. D. / IL-2 modulation of murine T-cell oncogene expression (42465). In: Proceedings of the Society for Experimental Biology and Medicine. 1987 ; Vol. 184, No. 2. pp. 186-190.
@article{8856a81b0ceb4ef2bdd3303206436c72,
title = "IL-2 modulation of murine T-cell oncogene expression (42465)",
abstract = "C-myb, a cellular oncogene associated with normal thymic development, was found to be highly expressed in four interleukin 2 (IL-2)-independent T-cell lines, but not in two of three IL-2-dependent T-cell lines. The IL-2-dependent lines, HT2 and CTLL-2, were found to have low levels of c-myb mRNA in the presence of IL-2. However, short-term IL-2 depletion resulted in at least fivefold increases in c-myb message. Add-back of IL-2 after 30 hr IL-2-depletion of CTLL-2 cells resulted in return to baseline low-level c-myb mRNA. Expression of the oncogenes myc, bas, raf, and abl as well as the T-cell genes Thy-1 and C(T)β did not parallel that of c-myb. These studies indicate that removal of a growth factor can result in increased levels of a specific cellular oncogene and that two nuclear protooncogenes (c-myb and c-myc) are expressed differentially during cell growth. These results may help to explain aspects of intrathymic T-cell differentiation where there is very high c-myb expression in the fact of limiting amounts of growth factors such as IL-2.",
author = "Seldin, {Michael F} and Mountz, {J. D.} and Mushinski, {J. F.} and Smith, {H. R.} and Steinberg, {A. D.}",
year = "1987",
language = "English (US)",
volume = "184",
pages = "186--190",
journal = "Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)",
issn = "1535-3702",
publisher = "Society for Experimental Biology and Medicine",
number = "2",

}

TY - JOUR

T1 - IL-2 modulation of murine T-cell oncogene expression (42465)

AU - Seldin, Michael F

AU - Mountz, J. D.

AU - Mushinski, J. F.

AU - Smith, H. R.

AU - Steinberg, A. D.

PY - 1987

Y1 - 1987

N2 - C-myb, a cellular oncogene associated with normal thymic development, was found to be highly expressed in four interleukin 2 (IL-2)-independent T-cell lines, but not in two of three IL-2-dependent T-cell lines. The IL-2-dependent lines, HT2 and CTLL-2, were found to have low levels of c-myb mRNA in the presence of IL-2. However, short-term IL-2 depletion resulted in at least fivefold increases in c-myb message. Add-back of IL-2 after 30 hr IL-2-depletion of CTLL-2 cells resulted in return to baseline low-level c-myb mRNA. Expression of the oncogenes myc, bas, raf, and abl as well as the T-cell genes Thy-1 and C(T)β did not parallel that of c-myb. These studies indicate that removal of a growth factor can result in increased levels of a specific cellular oncogene and that two nuclear protooncogenes (c-myb and c-myc) are expressed differentially during cell growth. These results may help to explain aspects of intrathymic T-cell differentiation where there is very high c-myb expression in the fact of limiting amounts of growth factors such as IL-2.

AB - C-myb, a cellular oncogene associated with normal thymic development, was found to be highly expressed in four interleukin 2 (IL-2)-independent T-cell lines, but not in two of three IL-2-dependent T-cell lines. The IL-2-dependent lines, HT2 and CTLL-2, were found to have low levels of c-myb mRNA in the presence of IL-2. However, short-term IL-2 depletion resulted in at least fivefold increases in c-myb message. Add-back of IL-2 after 30 hr IL-2-depletion of CTLL-2 cells resulted in return to baseline low-level c-myb mRNA. Expression of the oncogenes myc, bas, raf, and abl as well as the T-cell genes Thy-1 and C(T)β did not parallel that of c-myb. These studies indicate that removal of a growth factor can result in increased levels of a specific cellular oncogene and that two nuclear protooncogenes (c-myb and c-myc) are expressed differentially during cell growth. These results may help to explain aspects of intrathymic T-cell differentiation where there is very high c-myb expression in the fact of limiting amounts of growth factors such as IL-2.

UR - http://www.scopus.com/inward/record.url?scp=0023157855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023157855&partnerID=8YFLogxK

M3 - Article

C2 - 3492715

AN - SCOPUS:0023157855

VL - 184

SP - 186

EP - 190

JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

SN - 1535-3702

IS - 2

ER -