IL-17 markedly up-regulates β-defensin-2 expression in human airway epithelium via JAK and NF-κB signaling pathways

Cheng Yuan Kao, Yin Chen, Philip Thai, Shinichiro Wachi, Fei Huang, Christy Kim, Richart W Harper, Reen Wu

Research output: Contribution to journalArticle

313 Citations (Scopus)

Abstract

Using microarray gene expression analysis, we first observed a profound elevation of human β-defensin-2 (hBD-2) message in IL-17-treated primary human airway epithelial cells. Further comparison of this stimulation with a panel of cytokines (IL-1α, 1β, 2-13, and 15-18; IFN-γ; GM-CSF; and TNF-α demonstrated that IL-17 was the most potent cytokine to induce hBD-2 message (>75-fold). IL-17-induced stimulation of hBD-2 was time and dose dependent, and this stimulation also occurred at the protein level. Further studies demonstrated that hBD-2 stimulation was attenuated by IL-17R-specific Ab, but not by IL-1R antagonist or the neutralizing anti-IL-6 Ab. This suggests an IL-17R-mediated signaling pathway rather than an IL-17-induced IL-1αβ and/or IL-6 autocrine/paracrine loop. hBD-2 stimulation was sensitive to the inhibition of the JAK pathway, and to the inhibitors that affect NF-κB translocation and the DNA-binding activity of its p65 NF-κB subunit. Transient transfection of airway epithelial cells with an hBD-2 promoter-luciferase reporter gene expression construct demonstrated that IL-17 stimulated promoter-reporter gene activity, suggesting a transcriptional mechanism for hBD-2 induction. These results support an IL-17R-mediated signaling pathway involving JAK and NF-κB in the transcriptional stimulation of hBD-2 gene expression in airway epithelium. Because IL-17 has been identified in a number of airway diseases, especially diseases related to microbial infection, these findings provide a new insight into how IL-17 may play an important link between innate and adaptive immunity, thereby combating infection locally within the airway epithelium.

Original languageEnglish (US)
Pages (from-to)3482-3491
Number of pages10
JournalJournal of Immunology
Volume173
Issue number5
StatePublished - Sep 1 2004

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Defensins
Interleukin-17
Up-Regulation
Epithelium
Interleukin-1
Reporter Genes
Gene Expression
Interleukin-6
Epithelial Cells
Cytokines
Adaptive Immunity
Granulocyte-Macrophage Colony-Stimulating Factor
Infection
Luciferases
Innate Immunity
Transfection
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

IL-17 markedly up-regulates β-defensin-2 expression in human airway epithelium via JAK and NF-κB signaling pathways. / Kao, Cheng Yuan; Chen, Yin; Thai, Philip; Wachi, Shinichiro; Huang, Fei; Kim, Christy; Harper, Richart W; Wu, Reen.

In: Journal of Immunology, Vol. 173, No. 5, 01.09.2004, p. 3482-3491.

Research output: Contribution to journalArticle

Kao, Cheng Yuan ; Chen, Yin ; Thai, Philip ; Wachi, Shinichiro ; Huang, Fei ; Kim, Christy ; Harper, Richart W ; Wu, Reen. / IL-17 markedly up-regulates β-defensin-2 expression in human airway epithelium via JAK and NF-κB signaling pathways. In: Journal of Immunology. 2004 ; Vol. 173, No. 5. pp. 3482-3491.
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