Abstract
It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
Original language | English (US) |
---|---|
Pages (from-to) | 427-431 |
Number of pages | 5 |
Journal | Oncology Reports |
Volume | 9 |
Issue number | 2 |
State | Published - Mar 2002 |
Externally published | Yes |
Keywords
- Cytotoxicity
- Histamine
- IL-12
- IL-15
- IL-2
- Melanoma
- YAC-1
ASJC Scopus subject areas
- Oncology
- Cancer Research