IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells

Katarzyna Kozar, Rafal Kaminski, Adam Giermasz, Grzegorz Basak, Radoslaw Zagozdzon, Jolanta Rybczynska, Maria Wasik, Witold Lasek, Marek Jakóbisiak, Jakub Golab

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4 Scopus citations

Abstract

It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalOncology Reports
Volume9
Issue number2
StatePublished - Mar 2002
Externally publishedYes

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Keywords

  • Cytotoxicity
  • Histamine
  • IL-12
  • IL-15
  • IL-2
  • Melanoma
  • YAC-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kozar, K., Kaminski, R., Giermasz, A., Basak, G., Zagozdzon, R., Rybczynska, J., Wasik, M., Lasek, W., Jakóbisiak, M., & Golab, J. (2002). IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells. Oncology Reports, 9(2), 427-431.