Abstract
It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 427-431 |
| Number of pages | 5 |
| Journal | Oncology Reports |
| Volume | 9 |
| Issue number | 2 |
| State | Published - Mar 2002 |
| Externally published | Yes |
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Keywords
- Cytotoxicity
- Histamine
- IL-12
- IL-15
- IL-2
- Melanoma
- YAC-1
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells. / Kozar, Katarzyna; Kaminski, Rafal; Giermasz, Adam; Basak, Grzegorz; Zagozdzon, Radoslaw; Rybczynska, Jolanta; Wasik, Maria; Lasek, Witold; Jakóbisiak, Marek; Golab, Jakub.
In: Oncology Reports, Vol. 9, No. 2, 03.2002, p. 427-431.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells
AU - Kozar, Katarzyna
AU - Kaminski, Rafal
AU - Giermasz, Adam
AU - Basak, Grzegorz
AU - Zagozdzon, Radoslaw
AU - Rybczynska, Jolanta
AU - Wasik, Maria
AU - Lasek, Witold
AU - Jakóbisiak, Marek
AU - Golab, Jakub
PY - 2002/3
Y1 - 2002/3
N2 - It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
AB - It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
KW - Cytotoxicity
KW - Histamine
KW - IL-12
KW - IL-15
KW - IL-2
KW - Melanoma
KW - YAC-1
UR - http://www.scopus.com/inward/record.url?scp=0036517055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036517055&partnerID=8YFLogxK
M3 - Article
C2 - 11836621
AN - SCOPUS:0036517055
VL - 9
SP - 427
EP - 431
JO - Oncology Reports
JF - Oncology Reports
SN - 1021-335X
IS - 2
ER -