IgE reactivity against a cross-reactivity allergen in crustacea and mollusca

Evidence for tropomyosin as the common allergen

Patrick S Leung, Kuen Chow Wing Kuen Chow, S. Duffey, Shan Kwan Hoi Shan Kwan, M. Eric Gershwin, Hou Chu Ka Hou Chu

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Background: Although cross-reactivity between mollusks and other crustaceans in shrimp-sensitive subjects has been reported, the mechanism of this allergenic cross-reactivity has not been studied in detail. Objective: To investigate this cross-reactivity in vitro, we have taken advantage of a complementary DNA that expresses tropomyosin, the immunodominant shrimp allergen. Methods: Serum IgE from nine patients with known anaphylaxis to shrimp and five normal volunteers were analyzed by immunoblotting against 13 distinct crustaceans and mollusks. As additional antigens, muscle preparations were isolated from grasshopper, cockroach, fruit fly, chicken, and mouse. Results: Sera from all patients, but not control subjects, reacted specifically with a 38 kd protein in all crustaceans and mollusks studied. In addition, 8 of 9 sera from patients, but from none of the normal control subjects, recognized proteins of various other molecular weights among the mollusk extracts studied. The 38 kd protein was identified as tropomyosin and was shown to share immunodominant epitopes among all species of crustaceans and mollusks tested by specific absorption studies. Moreover, sera from all nine subjects with shrimp allergy demonstrated IgE reactivities against grasshopper, cockroach, and fruit fly but not chicken or murine muscle. Conclusion: The allergic epitopes on tropomyosin are conserved among invertebrates including not only shellfish but also insects. This latter observation suggests that persons sensitive to shrimp should undergo further study for potential cross-reactive inhalant or ingested insect sensitivity.

Original languageEnglish (US)
Pages (from-to)954-961
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume98
Issue number5
StatePublished - 1996

Fingerprint

Crustacea
Tropomyosin
Mollusca
Allergens
Immunoglobulin E
Cockroaches
Grasshoppers
Serum
Diptera
Insects
Chickens
Fruit
Shellfish
Immunodominant Epitopes
Muscles
Proteins
Anaphylaxis
Invertebrates
Immunoblotting
Epitopes

Keywords

  • cross-reactivity
  • crustaceans
  • IgE
  • insects
  • mollusks
  • tropomyosin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

IgE reactivity against a cross-reactivity allergen in crustacea and mollusca : Evidence for tropomyosin as the common allergen. / Leung, Patrick S; Wing Kuen Chow, Kuen Chow; Duffey, S.; Hoi Shan Kwan, Shan Kwan; Gershwin, M. Eric; Ka Hou Chu, Hou Chu.

In: Journal of Allergy and Clinical Immunology, Vol. 98, No. 5, 1996, p. 954-961.

Research output: Contribution to journalArticle

Leung, Patrick S ; Wing Kuen Chow, Kuen Chow ; Duffey, S. ; Hoi Shan Kwan, Shan Kwan ; Gershwin, M. Eric ; Ka Hou Chu, Hou Chu. / IgE reactivity against a cross-reactivity allergen in crustacea and mollusca : Evidence for tropomyosin as the common allergen. In: Journal of Allergy and Clinical Immunology. 1996 ; Vol. 98, No. 5. pp. 954-961.
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