Abstract
Background: Although cross-reactivity between mollusks and other crustaceans in shrimp-sensitive subjects has been reported, the mechanism of this allergenic cross-reactivity has not been studied in detail. Objective: To investigate this cross-reactivity in vitro, we have taken advantage of a complementary DNA that expresses tropomyosin, the immunodominant shrimp allergen. Methods: Serum IgE from nine patients with known anaphylaxis to shrimp and five normal volunteers were analyzed by immunoblotting against 13 distinct crustaceans and mollusks. As additional antigens, muscle preparations were isolated from grasshopper, cockroach, fruit fly, chicken, and mouse. Results: Sera from all patients, but not control subjects, reacted specifically with a 38 kd protein in all crustaceans and mollusks studied. In addition, 8 of 9 sera from patients, but from none of the normal control subjects, recognized proteins of various other molecular weights among the mollusk extracts studied. The 38 kd protein was identified as tropomyosin and was shown to share immunodominant epitopes among all species of crustaceans and mollusks tested by specific absorption studies. Moreover, sera from all nine subjects with shrimp allergy demonstrated IgE reactivities against grasshopper, cockroach, and fruit fly but not chicken or murine muscle. Conclusion: The allergic epitopes on tropomyosin are conserved among invertebrates including not only shellfish but also insects. This latter observation suggests that persons sensitive to shrimp should undergo further study for potential cross-reactive inhalant or ingested insect sensitivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 954-961 |
| Number of pages | 8 |
| Journal | Journal of Allergy and Clinical Immunology |
| Volume | 98 |
| Issue number | 5 |
| State | Published - 1996 |
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Keywords
- cross-reactivity
- crustaceans
- IgE
- insects
- mollusks
- tropomyosin
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
IgE reactivity against a cross-reactivity allergen in crustacea and mollusca : Evidence for tropomyosin as the common allergen. / Leung, Patrick S; Wing Kuen Chow, Kuen Chow; Duffey, S.; Hoi Shan Kwan, Shan Kwan; Gershwin, M. Eric; Ka Hou Chu, Hou Chu.
In: Journal of Allergy and Clinical Immunology, Vol. 98, No. 5, 1996, p. 954-961.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IgE reactivity against a cross-reactivity allergen in crustacea and mollusca
T2 - Evidence for tropomyosin as the common allergen
AU - Leung, Patrick S
AU - Wing Kuen Chow, Kuen Chow
AU - Duffey, S.
AU - Hoi Shan Kwan, Shan Kwan
AU - Gershwin, M. Eric
AU - Ka Hou Chu, Hou Chu
PY - 1996
Y1 - 1996
N2 - Background: Although cross-reactivity between mollusks and other crustaceans in shrimp-sensitive subjects has been reported, the mechanism of this allergenic cross-reactivity has not been studied in detail. Objective: To investigate this cross-reactivity in vitro, we have taken advantage of a complementary DNA that expresses tropomyosin, the immunodominant shrimp allergen. Methods: Serum IgE from nine patients with known anaphylaxis to shrimp and five normal volunteers were analyzed by immunoblotting against 13 distinct crustaceans and mollusks. As additional antigens, muscle preparations were isolated from grasshopper, cockroach, fruit fly, chicken, and mouse. Results: Sera from all patients, but not control subjects, reacted specifically with a 38 kd protein in all crustaceans and mollusks studied. In addition, 8 of 9 sera from patients, but from none of the normal control subjects, recognized proteins of various other molecular weights among the mollusk extracts studied. The 38 kd protein was identified as tropomyosin and was shown to share immunodominant epitopes among all species of crustaceans and mollusks tested by specific absorption studies. Moreover, sera from all nine subjects with shrimp allergy demonstrated IgE reactivities against grasshopper, cockroach, and fruit fly but not chicken or murine muscle. Conclusion: The allergic epitopes on tropomyosin are conserved among invertebrates including not only shellfish but also insects. This latter observation suggests that persons sensitive to shrimp should undergo further study for potential cross-reactive inhalant or ingested insect sensitivity.
AB - Background: Although cross-reactivity between mollusks and other crustaceans in shrimp-sensitive subjects has been reported, the mechanism of this allergenic cross-reactivity has not been studied in detail. Objective: To investigate this cross-reactivity in vitro, we have taken advantage of a complementary DNA that expresses tropomyosin, the immunodominant shrimp allergen. Methods: Serum IgE from nine patients with known anaphylaxis to shrimp and five normal volunteers were analyzed by immunoblotting against 13 distinct crustaceans and mollusks. As additional antigens, muscle preparations were isolated from grasshopper, cockroach, fruit fly, chicken, and mouse. Results: Sera from all patients, but not control subjects, reacted specifically with a 38 kd protein in all crustaceans and mollusks studied. In addition, 8 of 9 sera from patients, but from none of the normal control subjects, recognized proteins of various other molecular weights among the mollusk extracts studied. The 38 kd protein was identified as tropomyosin and was shown to share immunodominant epitopes among all species of crustaceans and mollusks tested by specific absorption studies. Moreover, sera from all nine subjects with shrimp allergy demonstrated IgE reactivities against grasshopper, cockroach, and fruit fly but not chicken or murine muscle. Conclusion: The allergic epitopes on tropomyosin are conserved among invertebrates including not only shellfish but also insects. This latter observation suggests that persons sensitive to shrimp should undergo further study for potential cross-reactive inhalant or ingested insect sensitivity.
KW - cross-reactivity
KW - crustaceans
KW - IgE
KW - insects
KW - mollusks
KW - tropomyosin
UR - http://www.scopus.com/inward/record.url?scp=0029853999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029853999&partnerID=8YFLogxK
M3 - Article
C2 - 8939159
AN - SCOPUS:0029853999
VL - 98
SP - 954
EP - 961
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 5
ER -