IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen

N. Orida, J. D. Feldman, D. H. Katz, Fu-Tong Liu

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Abstract

We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10-10 M DNP-BSA. Release of incorporated [3H]-serotonin did not always parellel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcRε-bearing cells are mediated via specific IgE bound to membrane FcRε.

Original languageEnglish (US)
Pages (from-to)2166-2171
Number of pages6
JournalJournal of Experimental Medicine
Volume157
Issue number6
StatePublished - 1983

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ASJC Scopus subject areas

  • Immunology

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Orida, N., Feldman, J. D., Katz, D. H., & Liu, F-T. (1983). IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. Journal of Experimental Medicine, 157(6), 2166-2171.