IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen

N. Orida, J. D. Feldman, D. H. Katz, Fu-Tong Liu

Research output: Contribution to journalArticle

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Abstract

We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10-10 M DNP-BSA. Release of incorporated [3H]-serotonin did not always parellel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcRε-bearing cells are mediated via specific IgE bound to membrane FcRε.

Original languageEnglish (US)
Pages (from-to)2166-2171
Number of pages6
JournalJournal of Experimental Medicine
Volume157
Issue number6
StatePublished - 1983

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Chemotaxis
Immunoglobulin E
Leukemia
Antigens
Basophils
Serotonin
Membranes
dinitrophenyl-bovine serum albumin

ASJC Scopus subject areas

  • Immunology

Cite this

Orida, N., Feldman, J. D., Katz, D. H., & Liu, F-T. (1983). IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. Journal of Experimental Medicine, 157(6), 2166-2171.

IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. / Orida, N.; Feldman, J. D.; Katz, D. H.; Liu, Fu-Tong.

In: Journal of Experimental Medicine, Vol. 157, No. 6, 1983, p. 2166-2171.

Research output: Contribution to journalArticle

Orida, N, Feldman, JD, Katz, DH & Liu, F-T 1983, 'IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen', Journal of Experimental Medicine, vol. 157, no. 6, pp. 2166-2171.
Orida, N. ; Feldman, J. D. ; Katz, D. H. ; Liu, Fu-Tong. / IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. In: Journal of Experimental Medicine. 1983 ; Vol. 157, No. 6. pp. 2166-2171.
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