Abstract
We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10-10 M DNP-BSA. Release of incorporated [3H]-serotonin did not always parellel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcRε-bearing cells are mediated via specific IgE bound to membrane FcRε.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2166-2171 |
| Number of pages | 6 |
| Journal | Journal of Experimental Medicine |
| Volume | 157 |
| Issue number | 6 |
| State | Published - 1983 |
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ASJC Scopus subject areas
- Immunology
Cite this
IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. / Orida, N.; Feldman, J. D.; Katz, D. H.; Liu, Fu-Tong.
In: Journal of Experimental Medicine, Vol. 157, No. 6, 1983, p. 2166-2171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen
AU - Orida, N.
AU - Feldman, J. D.
AU - Katz, D. H.
AU - Liu, Fu-Tong
PY - 1983
Y1 - 1983
N2 - We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10-10 M DNP-BSA. Release of incorporated [3H]-serotonin did not always parellel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcRε-bearing cells are mediated via specific IgE bound to membrane FcRε.
AB - We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10-10 M DNP-BSA. Release of incorporated [3H]-serotonin did not always parellel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcRε-bearing cells are mediated via specific IgE bound to membrane FcRε.
UR - http://www.scopus.com/inward/record.url?scp=0020620948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020620948&partnerID=8YFLogxK
M3 - Article
C2 - 6189956
AN - SCOPUS:0020620948
VL - 157
SP - 2166
EP - 2171
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 6
ER -