We show that IgE-binding protein (εBP) is found primarily in the cytoplasm of rat basophilic leukemia (RBL) cells and COS-1 cells transfected with εBP cDNA. Antibodies to a synthetic peptide internal to εBP were generated that specifically recognized εBP by protein immunoblotting. These antibodies also bind the surface of RBL cells. Surprisingly, blot hybridization analysis of RNA from nine various normal rat tissues showed that the εBP gene is transcribed in all the tissues tested as well as in a mouse macrophage-like cell line.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - 1988|
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