Idiopathic microscopic colitis of rhesus macaques: Quantitative assessment of colonic mucosa

Amir Ardeshir, Karen L. Oslund, Frank Ventimiglia, Joann Yee, Nicholas W. Lerche, Dallas M. Hyde

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Idiopathic chronic diarrhea (ICD) is a common cause of morbidity and mortality among juvenile rhesus macaques. While lesions may be absent at colonoscopy, the histopathologic evaluation of the biopsy specimens is consistent with human macroscopic colitis (MC). In this study, we developed an isotropic uniform random sampling method to evaluate macroscopic and microscopic changes and applied it on proximal ascending colon in monkeys. Colonic tissue and peripheral blood specimens were collected from six MC and six control juvenile macaques at necropsy. Uniform random samples were collected from the colon using punch biopsy tools. The volume of epithelium and lamina propria were estimated in thick (25 μm) sections using point probes and normalized to the area of muscularis mucosae. Our data suggests a significant increase of the Vs of the lamina propria (1.9-fold, P = 0.02) and epithelium (1.4-fold, P = 0.05) in subjects with MC. The average colonic surface mucosa area in the MC monkeys increased 1.4-fold over the controls (P = 0.02). The volume of the proximal colon in animals with MC showed a 2.4-fold increase over the non-diarrhea control monkeys (P = 0.0001). Cytokine, chemokine, and growth factor levels in peripheral blood were found to be correlated with the volume estimate of the lamina propria and epithelium. We found that ICD in macaques has features which simulates human MC and can be used as a spontaneous animal model for human MC. Furthermore, this developed sampling method can be used for unbiased preclinical evaluation of therapeutics in this animal model.

Original languageEnglish (US)
Pages (from-to)1169-1179
Number of pages11
JournalAnatomical Record
Volume296
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Microscopic Colitis
colitis
Colitis
Macaca mulatta
mucosa
Mucous Membrane
fold
laminae (animals)
animal
colon
Haplorhini
monkeys
blood
epithelium
Epithelium
Macaca
sampling
morbidity
biopsy
Diarrhea

Keywords

  • Colon
  • Inflammatory bowel disease
  • Microscopic colitis
  • Rhesus macaques
  • Stereology

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology

Cite this

Ardeshir, A., Oslund, K. L., Ventimiglia, F., Yee, J., Lerche, N. W., & Hyde, D. M. (2013). Idiopathic microscopic colitis of rhesus macaques: Quantitative assessment of colonic mucosa. Anatomical Record, 296(8), 1169-1179. https://doi.org/10.1002/ar.22727

Idiopathic microscopic colitis of rhesus macaques : Quantitative assessment of colonic mucosa. / Ardeshir, Amir; Oslund, Karen L.; Ventimiglia, Frank; Yee, Joann; Lerche, Nicholas W.; Hyde, Dallas M.

In: Anatomical Record, Vol. 296, No. 8, 08.2013, p. 1169-1179.

Research output: Contribution to journalArticle

Ardeshir, A, Oslund, KL, Ventimiglia, F, Yee, J, Lerche, NW & Hyde, DM 2013, 'Idiopathic microscopic colitis of rhesus macaques: Quantitative assessment of colonic mucosa', Anatomical Record, vol. 296, no. 8, pp. 1169-1179. https://doi.org/10.1002/ar.22727
Ardeshir A, Oslund KL, Ventimiglia F, Yee J, Lerche NW, Hyde DM. Idiopathic microscopic colitis of rhesus macaques: Quantitative assessment of colonic mucosa. Anatomical Record. 2013 Aug;296(8):1169-1179. https://doi.org/10.1002/ar.22727
Ardeshir, Amir ; Oslund, Karen L. ; Ventimiglia, Frank ; Yee, Joann ; Lerche, Nicholas W. ; Hyde, Dallas M. / Idiopathic microscopic colitis of rhesus macaques : Quantitative assessment of colonic mucosa. In: Anatomical Record. 2013 ; Vol. 296, No. 8. pp. 1169-1179.
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