Identifying peptide ligands for cell surface receptors using cell-growth-on-bead assay and one-bead one-compound combinatorial library

Derick H Lau, Linlang Guo, Ruiwu Liu, Aimin Song, Chunkui Shao, Kit Lam

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

A cell-growth-on-bead assay was used to screen one-bead, one-compound combinatorial libraries for peptide ligands that attract attachment and growth of human lung cancer cells. A consensus cyclic peptide sequence, D-cys-L-asn-L-gly-L-arg-L-gly-L-glu-L-gln-D-cyS (cNGRGEQc), was identified as a ligand for a cell surface receptor, α3β1 integrin. This peptide ligand and its analogs have the potential to be used as diagnostic and therapeutic agents for lung cancer.

Original languageEnglish (US)
Pages (from-to)497-500
Number of pages4
JournalBiotechnology Letters
Volume24
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Cell-growth-on-bead assay
  • Integrin
  • Lung cancer
  • One-bead one-compound combinatorial library
  • Peptide ligand

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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