Identification of VPS13C as a galectin-12- binding protein that regulates galectin-12 protein stability and adipogenesis

Ri Yao Yang, Huiting Xue, Lan Yu, Antonio Velayos-Baeza, Anthony P. Monaco, Fu-Tong Liu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Galectin-12, a member of the galectin family of β-galactoside-binding animal lectins, is preferentially expressed in adipocytes and required for adipocyte differentiation in vitro. This protein was recently found to regulate lipolysis, whole body adiposity, and glucose homeostasis in vivo. Here we identify VPS13C, a member of the VPS13 family of vacuolar protein sorting-associated proteins highly conserved throughout eukaryotic evolution, as a major galectin-12-binding protein. VPS13C is upregulated during adipocyte differentiation, and is required for galectin-12 protein stability. Knockdown of Vps13c markedly reduces the steady-state levels of galectin-12 by promoting its degradation through primarily the lysosomal pathway, and impairs adipocyte differentiation. Our studies also suggest that VPS13C may have a broader role in protein quality control. The regulation of galectin-12 stability by VPS13C could potentially be exploited for therapeutic intervention of obesity and related metabolic diseases.

Original languageEnglish (US)
Article numbere0153534
JournalPLoS One
Volume11
Issue number4
DOIs
StatePublished - Apr 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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