Identification of two novel coccidian species shed by California Sea Lions (Zalophus californianus)

Daphne Carlson-Bremer, Christine K Johnson, Robin H. Miller, Frances M D Gulland, Patricia A Conrad, James D. Wasmuth, Kathleen M. Colegrove, Michael E. Grigg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Routine fecal examination revealed novel coccidian oocysts in asymptomatic California sea lions (Zalophus californianus) in a rehabilitation facility. Coccidian oocysts were observed in fecal samples collected from 15 of 410 California sea lions admitted to The Marine Mammal Center between April 2007 and October 2009. Phylogenetic analysis using the full ITS-1 region, partial small subunit 18S rDNA sequence, and the Apicomplexa rpoB region identified 2 distinct sequence clades, referred to as Coccidia A and Coccidia B, and placed them in the Sarcocystidae, grouped with the tissue-cystforming coccidia. Both sequence clades resolved as individual taxa at ITS-1 and rpoB and were most closely related to Neospora caninum. Coccidia A was identified in 11 and Coccidia B in 4 of 12 sea lion oocyst samples successfully sequenced (3 of those sea lions were co-infected with both parasites). Shedding of Coccidia A oocysts was not associated with age class, sex, or stranding location, but yearlings represented the majority of shedders (8/15). This is the first study to use molecular phylogenetics to identify and describe coccidian parasites shed by a marine mammal.

Original languageEnglish (US)
Pages (from-to)347-354
Number of pages8
JournalJournal of Parasitology
Volume98
Issue number2
DOIs
StatePublished - Apr 2012

Fingerprint

Sea Lions
Coccidia
pinniped
Oocysts
oocysts
marine mammal
parasite
Otariidae
marine mammals
phylogenetics
stranding
Mammals
Sarcocystidae
Parasites
age class
Neospora
Apicomplexa
parasites
Neospora caninum
phylogeny

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Identification of two novel coccidian species shed by California Sea Lions (Zalophus californianus). / Carlson-Bremer, Daphne; Johnson, Christine K; Miller, Robin H.; Gulland, Frances M D; Conrad, Patricia A; Wasmuth, James D.; Colegrove, Kathleen M.; Grigg, Michael E.

In: Journal of Parasitology, Vol. 98, No. 2, 04.2012, p. 347-354.

Research output: Contribution to journalArticle

Carlson-Bremer, D, Johnson, CK, Miller, RH, Gulland, FMD, Conrad, PA, Wasmuth, JD, Colegrove, KM & Grigg, ME 2012, 'Identification of two novel coccidian species shed by California Sea Lions (Zalophus californianus)', Journal of Parasitology, vol. 98, no. 2, pp. 347-354. https://doi.org/10.1645/GE-2752.1
Carlson-Bremer, Daphne ; Johnson, Christine K ; Miller, Robin H. ; Gulland, Frances M D ; Conrad, Patricia A ; Wasmuth, James D. ; Colegrove, Kathleen M. ; Grigg, Michael E. / Identification of two novel coccidian species shed by California Sea Lions (Zalophus californianus). In: Journal of Parasitology. 2012 ; Vol. 98, No. 2. pp. 347-354.
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