Identification of toxigenic Pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation.

G. J. Eamens, P. D. Kirkland, M. J. Turner, Ian Gardner, M. P. White, C. L. Hornitzky

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.

Original languageEnglish (US)
Pages (from-to)120-123
Number of pages4
JournalAustralian Veterinary Journal
Volume65
Issue number4
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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