Identification of the promoter and first exon of the mouse α1(III) collagen gene

G. Liau, Maria Mudryj, B. De Crombrugghe

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We have identified the promoter-proximal exon of the mouse α1(III) collagen gene using a synthetic oligonucleotide as a hybridization probe and have determined the DNA sequence of this exon and 380 base pairs 5' to it. The exact start site of transcription was localized with a primer extension experiment. The region upstream of the start of transcription shows only scattered homologies with the analogous sequences in the α1(I) and α2(I) mouse collagen genes although these genes are often co-expressed and co-regulated. The most striking homology with the type I gene is seen around the start of translation. This region contains an inverted repeat which could form a stem-loop structure with a calculated ΔG of -30 kcal in the type III collagen mRNA. When compared to the α1(I) and α2(I) signal peptides, the signal peptide of mouse α1(III) collagen presents less homology than when these segments are compared to each other.

Original languageEnglish (US)
Pages (from-to)3773-3777
Number of pages5
JournalJournal of Biological Chemistry
Volume260
Issue number6
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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