Identification of the etiologic agent of epizootic bovine abortion in field-collected Ornithodoros coriaceus Koch ticks

Ching I. Chen, Donald P. King, Myra Blanchard, Mark R. Hall, Brian M. Aldridge, Lizabeth Bowen, Jeffrey L Stott

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Epizootic bovine abortion (EBA), or foothill abortion as it has often been termed, is a tick-borne disease of pregnant cattle recognized in California, Nevada and Oregon. The primary objective of this study was to better define the relationship of a novel deltaproteobacterium, the putative etiological agent of EBA (aoEBA), with the Pajaroello tick (Ornithodoros coriaceus Koch), the recognized vector of EBA. Three developmental stages of O. coriaceus (larva, nymph, and adult) were collected from five locations in California, Nevada and Oregon. A polymerase chain reaction (PCR), developed for detection of aoEBA, was applied to DNA extracted from ticks. Southern blotting of the PCR products increased the number of ticks determined to be carrying the bacteria by seven-fold, suggesting the majority of infected ticks carry relatively low numbers of the pathogen. An effort was made to determine if an artificial blood meal would stimulate replication of the bacterial pathogen, thereby increasing the frequency in which aoEBA could be identified; no statistically significant effect was evident. The number of ticks determined to be carrying aoEBA varied with geographic location and ranged from 5 to 20%. aoEBA was found in both adults (12% of the males and 12% of the females) and nymphs (13%) but not larvae. Comparative analysis of dissected ticks provided strong evidence that the salivary gland was the most common location of aoEBA in field-collected ticks. No significant correlations were identified between the frequency of infection and tick weight, suggesting that increasing tick age and increased number of blood meals did not increase infectivity.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalVeterinary Microbiology
Volume120
Issue number3-4
DOIs
StatePublished - Mar 10 2007

Fingerprint

Ornithodoros coriaceus
Ornithodoros
abortion (animals)
Ticks
ticks
cattle
Nymph
blood meal
nymphs
Larva
Meals
Deltaproteobacteria
polymerase chain reaction
Tick-Borne Diseases
delta-Proteobacteria
Blood Substitutes
Geographic Locations
tick-borne diseases
Polymerase Chain Reaction
pathogens

Keywords

  • Argasidae
  • Deltaproteobacterium
  • Epizootic bovine abortion
  • Foothill abortion
  • Ornithodoros coriaceus
  • Pajaroello tick

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Identification of the etiologic agent of epizootic bovine abortion in field-collected Ornithodoros coriaceus Koch ticks. / Chen, Ching I.; King, Donald P.; Blanchard, Myra; Hall, Mark R.; Aldridge, Brian M.; Bowen, Lizabeth; Stott, Jeffrey L.

In: Veterinary Microbiology, Vol. 120, No. 3-4, 10.03.2007, p. 320-327.

Research output: Contribution to journalArticle

Chen, Ching I. ; King, Donald P. ; Blanchard, Myra ; Hall, Mark R. ; Aldridge, Brian M. ; Bowen, Lizabeth ; Stott, Jeffrey L. / Identification of the etiologic agent of epizootic bovine abortion in field-collected Ornithodoros coriaceus Koch ticks. In: Veterinary Microbiology. 2007 ; Vol. 120, No. 3-4. pp. 320-327.
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