Abstract
Using a 'one-bead one-peptide' combinatorial peptide library method, we have been able to identify peptide ligands that interact specifically with various macromolecular targets such as monoclonal antibodies, streptavidin, avidin, MHC-Class molecules, proteases, growth factor receptors, and gpIIb/IIIa integrin. In this paper, we test the hypothesis that small peptides that interact specifically with a small organic molecule can also be identified using this combinatorial peptide method. Using a small organic dye molecule, indigo carmine, as a color probe to screen a random L-heptapeptide and two D-hexa and octapeptide libraries, we were able to identify a specific peptide binding motif. Potential applications of this technology are described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-160 |
| Number of pages | 4 |
| Journal | Drug Development Research |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- Carmine
- Peptides
- Split synthesis
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Pharmacology
Cite this
Identification of small peptides that interact specifically with a small organic dye. / Lam, Kit; Zhao, Z. G.; Wade, S.; Krchnak, V.; Lebl, M.
In: Drug Development Research, Vol. 33, No. 2, 1994, p. 157-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of small peptides that interact specifically with a small organic dye
AU - Lam, Kit
AU - Zhao, Z. G.
AU - Wade, S.
AU - Krchnak, V.
AU - Lebl, M.
PY - 1994
Y1 - 1994
N2 - Using a 'one-bead one-peptide' combinatorial peptide library method, we have been able to identify peptide ligands that interact specifically with various macromolecular targets such as monoclonal antibodies, streptavidin, avidin, MHC-Class molecules, proteases, growth factor receptors, and gpIIb/IIIa integrin. In this paper, we test the hypothesis that small peptides that interact specifically with a small organic molecule can also be identified using this combinatorial peptide method. Using a small organic dye molecule, indigo carmine, as a color probe to screen a random L-heptapeptide and two D-hexa and octapeptide libraries, we were able to identify a specific peptide binding motif. Potential applications of this technology are described.
AB - Using a 'one-bead one-peptide' combinatorial peptide library method, we have been able to identify peptide ligands that interact specifically with various macromolecular targets such as monoclonal antibodies, streptavidin, avidin, MHC-Class molecules, proteases, growth factor receptors, and gpIIb/IIIa integrin. In this paper, we test the hypothesis that small peptides that interact specifically with a small organic molecule can also be identified using this combinatorial peptide method. Using a small organic dye molecule, indigo carmine, as a color probe to screen a random L-heptapeptide and two D-hexa and octapeptide libraries, we were able to identify a specific peptide binding motif. Potential applications of this technology are described.
KW - Carmine
KW - Peptides
KW - Split synthesis
UR - http://www.scopus.com/inward/record.url?scp=0027994775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027994775&partnerID=8YFLogxK
U2 - 10.1002/ddr.430330212
DO - 10.1002/ddr.430330212
M3 - Article
VL - 33
SP - 157
EP - 160
JO - Drug Development Research
JF - Drug Development Research
SN - 0272-4391
IS - 2
ER -