Identification of small peptides that interact specifically with a small organic dye

Kit Lam, Z. G. Zhao, S. Wade, V. Krchnak, M. Lebl

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Using a 'one-bead one-peptide' combinatorial peptide library method, we have been able to identify peptide ligands that interact specifically with various macromolecular targets such as monoclonal antibodies, streptavidin, avidin, MHC-Class molecules, proteases, growth factor receptors, and gpIIb/IIIa integrin. In this paper, we test the hypothesis that small peptides that interact specifically with a small organic molecule can also be identified using this combinatorial peptide method. Using a small organic dye molecule, indigo carmine, as a color probe to screen a random L-heptapeptide and two D-hexa and octapeptide libraries, we were able to identify a specific peptide binding motif. Potential applications of this technology are described.

Original languageEnglish (US)
Pages (from-to)157-160
Number of pages4
JournalDrug Development Research
Volume33
Issue number2
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • Carmine
  • Peptides
  • Split synthesis

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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