Abstract
Using a 'one-bead one-peptide' combinatorial peptide library method, we have been able to identify peptide ligands that interact specifically with various macromolecular targets such as monoclonal antibodies, streptavidin, avidin, MHC-Class molecules, proteases, growth factor receptors, and gpIIb/IIIa integrin. In this paper, we test the hypothesis that small peptides that interact specifically with a small organic molecule can also be identified using this combinatorial peptide method. Using a small organic dye molecule, indigo carmine, as a color probe to screen a random L-heptapeptide and two D-hexa and octapeptide libraries, we were able to identify a specific peptide binding motif. Potential applications of this technology are described.
Original language | English (US) |
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Pages (from-to) | 157-160 |
Number of pages | 4 |
Journal | Drug Development Research |
Volume | 33 |
Issue number | 2 |
DOIs | |
State | Published - 1994 |
Externally published | Yes |
Keywords
- Carmine
- Peptides
- Split synthesis
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Pharmacology