Identification of potential on-farm sources of Listeria monocytogenes in herds of dairy cattle

Hussni O. Mohammed, Korana Stipetic, Patrick L. McDonough, Ruben N. Gonzalez, Daryl V. Nydam, Edward R Atwill

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective - To elucidate the ecology of Listeria monocytogenes on dairy cattle farms by determining the prevalence of the organism in various samples. Sample Population - Dairy cattle operations in central New York State. Procedures - A repeated cross-sectional study design was used. Various samples were obtained from cattle (feces, composite udder milk, and udders), their environment (silage, feed bunks, water troughs, and floor bedding), inline milk filters, and bulk tank milk from 50 dairy farms. Samples were tested for L monocytogenes by use of a PCR assay with 2 steps of bacterial enrichment. Data were analyzed with mixed-effect logistic regression to control for the potential clustering of L monocytogenes on particular farms. Results - L monocytogenes was detected in composite milk, udder swab samples, and fecal samples at prevalences of 13%, 19%, and 43%, respectively. There was no significant clustering of the pathogen by farm. Listeria monocytogenes was more common in samples obtained from cattle and the environment during winter and summer versus the fall. The prevalence of L monocytogenes was twice as high in samples obtained from feed bunks, water troughs, and bedding, compared with that in samples obtained from silage (65%, 66%, 55%, and 30%, respectively). Conclusions and Clinical Relevance - L monocytogenes was more prevalent in samples obtained from dairy cattle and their environment than in milk samples. Strategies to control the pathogen in dairy operations should focus on cow hygiene and sanitary milk harvesting on the farm.

Original languageEnglish (US)
Pages (from-to)383-388
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume70
Issue number3
DOIs
StatePublished - Mar 2009

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Listeria monocytogenes
dairy cattle
Milk
herds
farms
Animal Mammary Glands
Silage
milk
sampling
Cluster Analysis
udders
water troughs
feed troughs
Water
dairy farming
silage
Ecology
Hygiene
Feces
Farms

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Identification of potential on-farm sources of Listeria monocytogenes in herds of dairy cattle. / Mohammed, Hussni O.; Stipetic, Korana; McDonough, Patrick L.; Gonzalez, Ruben N.; Nydam, Daryl V.; Atwill, Edward R.

In: American Journal of Veterinary Research, Vol. 70, No. 3, 03.2009, p. 383-388.

Research output: Contribution to journalArticle

Mohammed, Hussni O. ; Stipetic, Korana ; McDonough, Patrick L. ; Gonzalez, Ruben N. ; Nydam, Daryl V. ; Atwill, Edward R. / Identification of potential on-farm sources of Listeria monocytogenes in herds of dairy cattle. In: American Journal of Veterinary Research. 2009 ; Vol. 70, No. 3. pp. 383-388.
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