Identification of Piwil2-like (PL2L) proteins that promote tumorigenesis

Yin Ye, De Tao Yin, Li Chen, Quansheng Zhou, Rulong Shen, Gang He, Qingtao Yan, Zhenyu Tong, Andrew C. Issekutz, Charles L. Shapiro, Sanford H. Barsky, Haifan Lin, Jian-Jian Li, Jian Xin Gao

Research output: Contribution to journalArticle

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Abstract

PIWIL2, a member of PIWI/AGO gene family, is expressed in the germline stem cells (GSCs) of testis for gametogenesis but not in adult somatic and stem cells. It has been implicated to play an important role in tumor development. We have previously reported that precancerous stem cells (pCSCs) constitutively express Piwil2 transcripts to promote their proliferation. Here we show that these transcripts de facto represent Piwil2-like (PL2L) proteins. We have identified several PL2L proteins including PL2L80, PL2L60, PL2L50 and PL2L40, using combined methods of Gene-Exon-Mapping Reverse Transcription Polymerase Chain Reaction (GEM RT-PCR), bioinformatics and a group of novel monoclonal antibodies. Among them, PL2L60 rather than Piwil2 and other PL2L proteins is predominantly expressed in various types of human and mouse tumor cells. It promotes tumor cell survival and proliferation in vitro through up-regulation of Stat3 and Bcl2 gene expressions, the cell cycle entry from G0/1 into S-phase, and the nuclear expression of NF-kB, which contribute to the tumorigenicity of tumor cells in vivo. Consistently, PL2L proteins rather than Piwil2 are predominantly expressed in the cytoplasm or cytoplasm and nucleus of euchromatin-enriched tumor cells in human primary and metastatic cancers, such as breast and cervical cancers. Moreover, nuclear PL2L proteins are always co-expressed with nuclear NF-kB. These results reveal that PL2L60 can coordinate with NF-kB to promote tumorigenesis and might mediate a common pathway for tumor development without tissue restriction. The identification of PL2L proteins provides a novel insight into the mechanisms of cancer development as well as a novel bridge linking cancer diagnostics and anticancer drug development.

Original languageEnglish (US)
Article numbere13406
JournalPLoS One
Volume5
Issue number10
DOIs
StatePublished - 2010

Fingerprint

carcinogenesis
Tumors
Carcinogenesis
transcription factor NF-kappa B
Cells
NF-kappa B
Stem cells
neoplasms
stem cells
Neoplasms
Proteins
proteins
cytoplasm
Genes
uterine cervical neoplasms
antineoplastic agents
Polymerase chain reaction
Cytoplasm
gametogenesis
Stem Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ye, Y., Yin, D. T., Chen, L., Zhou, Q., Shen, R., He, G., ... Gao, J. X. (2010). Identification of Piwil2-like (PL2L) proteins that promote tumorigenesis. PLoS One, 5(10), [e13406]. https://doi.org/10.1371/journal.pone.0013406

Identification of Piwil2-like (PL2L) proteins that promote tumorigenesis. / Ye, Yin; Yin, De Tao; Chen, Li; Zhou, Quansheng; Shen, Rulong; He, Gang; Yan, Qingtao; Tong, Zhenyu; Issekutz, Andrew C.; Shapiro, Charles L.; Barsky, Sanford H.; Lin, Haifan; Li, Jian-Jian; Gao, Jian Xin.

In: PLoS One, Vol. 5, No. 10, e13406, 2010.

Research output: Contribution to journalArticle

Ye, Y, Yin, DT, Chen, L, Zhou, Q, Shen, R, He, G, Yan, Q, Tong, Z, Issekutz, AC, Shapiro, CL, Barsky, SH, Lin, H, Li, J-J & Gao, JX 2010, 'Identification of Piwil2-like (PL2L) proteins that promote tumorigenesis', PLoS One, vol. 5, no. 10, e13406. https://doi.org/10.1371/journal.pone.0013406
Ye, Yin ; Yin, De Tao ; Chen, Li ; Zhou, Quansheng ; Shen, Rulong ; He, Gang ; Yan, Qingtao ; Tong, Zhenyu ; Issekutz, Andrew C. ; Shapiro, Charles L. ; Barsky, Sanford H. ; Lin, Haifan ; Li, Jian-Jian ; Gao, Jian Xin. / Identification of Piwil2-like (PL2L) proteins that promote tumorigenesis. In: PLoS One. 2010 ; Vol. 5, No. 10.
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abstract = "PIWIL2, a member of PIWI/AGO gene family, is expressed in the germline stem cells (GSCs) of testis for gametogenesis but not in adult somatic and stem cells. It has been implicated to play an important role in tumor development. We have previously reported that precancerous stem cells (pCSCs) constitutively express Piwil2 transcripts to promote their proliferation. Here we show that these transcripts de facto represent Piwil2-like (PL2L) proteins. We have identified several PL2L proteins including PL2L80, PL2L60, PL2L50 and PL2L40, using combined methods of Gene-Exon-Mapping Reverse Transcription Polymerase Chain Reaction (GEM RT-PCR), bioinformatics and a group of novel monoclonal antibodies. Among them, PL2L60 rather than Piwil2 and other PL2L proteins is predominantly expressed in various types of human and mouse tumor cells. It promotes tumor cell survival and proliferation in vitro through up-regulation of Stat3 and Bcl2 gene expressions, the cell cycle entry from G0/1 into S-phase, and the nuclear expression of NF-kB, which contribute to the tumorigenicity of tumor cells in vivo. Consistently, PL2L proteins rather than Piwil2 are predominantly expressed in the cytoplasm or cytoplasm and nucleus of euchromatin-enriched tumor cells in human primary and metastatic cancers, such as breast and cervical cancers. Moreover, nuclear PL2L proteins are always co-expressed with nuclear NF-kB. These results reveal that PL2L60 can coordinate with NF-kB to promote tumorigenesis and might mediate a common pathway for tumor development without tissue restriction. The identification of PL2L proteins provides a novel insight into the mechanisms of cancer development as well as a novel bridge linking cancer diagnostics and anticancer drug development.",
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