Identification of oocyte progenitor cells in the zebrafish ovary

Bruce W. Draper

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Zebrafish breed year round and females are capable of producing thousands of eggs during their lifetime. This amazing fecundity is due to the fact that the adult ovary, contains premeiotic oocyte progenitor cells, called oogonia, which produce a continuous supply of new oocytes throughout adult life. Oocyte progenitor cells can be easily identified based on their expression of Vasa, and their characteristic nuclear morphology. Thus, the zebrafish ovary provides a unique and powerful system to study the genetic regulation of oocyte production in a vertebrate animal. A method is presented here for identifying oocyte progenitor cells in the zebrafish ovary using whole-mount confocal immunofluorescence that is simple and accurate.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages157-165
Number of pages9
Volume916
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume916
ISSN (Print)10643745

Keywords

  • Confocal immunofluorescence
  • Germ cell
  • Oocyte progenitors
  • Oogonia
  • Ovary
  • Vasa
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Draper, B. W. (2012). Identification of oocyte progenitor cells in the zebrafish ovary. In Methods in Molecular Biology (Vol. 916, pp. 157-165). (Methods in Molecular Biology; Vol. 916). https://doi.org/10.1007/978-1-61779-980-8-12