Identification of novel Ack1-interacting proteins and Ack1 phosphorylated sites in mouse brain by mass spectrometry

Maria del Mar Masdeu, Beatriz G. Armendáriz, Anna La Torre Vila, Eduardo Soriano, Ferran Burgaya, Jesús Mariano Ureña

Research output: Contribution to journalArticle

Abstract

Ack1 (activated Cdc42-associated tyrosine kinase) is a non-receptor tyrosine kinase that is highly expressed in brain. This kinase contains several protein-protein interaction domains and its action is partially regulated by phosphorylation. As a first step to address the neuronal functions of Ack1, here we screened mouse brain samples to identify proteins that interact with this kinase. Using mass spectrometry analysis, we identified new putative partners for Ack1 including cytoskeletal proteins such as Drebrin or MAP4; adhesion regulators such as NCAM1 and neurabin-2; and synapse mediators such as SynGAP, GRIN1 and GRIN3. In addition, we confirmed that Ack1 and CAMKII both co-immunoprecipitate and co-localize in neurons. We also identified that adult and P5 samples contained the phosphorylated residues Thr 104 and Ser 825, and only P5 samples contained phosphorylated Ser 722, a site linked to cancer and interleukin signaling when phosphorylated. All these findings support the notion that Ack1 could be involved in neuronal plasticity.

Original languageEnglish (US)
Pages (from-to)101146-101157
Number of pages12
JournalOncotarget
Volume8
Issue number60
DOIs
StatePublished - Jan 1 2017

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Keywords

  • Ack1
  • Central nervous system
  • Development
  • Tyrosine kinase

ASJC Scopus subject areas

  • Oncology

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