Identification of Neospora caninum in aborted bovine fetuses of Southern Chile

Manuel Moroni, Mauricio A. Navarro, Enrique Paredes, Alex Romero, Aintzane Alberdi, Tania Lischinsky, Dadin P. Moore, Carlos M. Campero, Francisco A Uzal

Research output: Contribution to journalArticle

Abstract

The aim of this study was to identify Neospora caninum in histologic sections of brain and optic nerve of aborted bovine fetuses. Sections of these tissues from 296 aborted bovine fetuses from Southern Chile, submitted between 2000 and 2010, to the Instituto de Patología Animal of the Universidad Austral de Chile were analyzed. Forty-four (14.9%) fetuses had microscopic lesions compatible with bovine neosporosis, including gliosis, necrosis, intralesional protozoan cysts and neuritis of optic nerves. N. caninum was identified by immunohistochemistry in brain sections of 27 cases (9.1%) with compatible lesions. Positive immunostaining was also found in the optic nerves of 13 cases (4.4%) with neuritis. N. caninum DNA was identified by PCR in brain sections in 31 cases (10.5%). In total, 36 cases (12.2%) were positive by either technique. Phylogenetic analysis of the ITS1 gene sequence of N. caninum revealed a high degree of conservation among different isolates. This is the first report of N. caninum-associated optic neuritis in cattle and also the first report of confirmed bovine abortion associated with this parasite in Chile.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalBrazilian Journal of Veterinary Pathology
Volume11
Issue number2
DOIs
StatePublished - Jul 1 2018

Keywords

  • Bovine abortion
  • Immunohistochemistry
  • Neospora caninum
  • PCR
  • Phylogenetic analysis

ASJC Scopus subject areas

  • veterinary (miscalleneous)

Fingerprint Dive into the research topics of 'Identification of Neospora caninum in aborted bovine fetuses of Southern Chile'. Together they form a unique fingerprint.

  • Cite this

    Moroni, M., Navarro, M. A., Paredes, E., Romero, A., Alberdi, A., Lischinsky, T., Moore, D. P., Campero, C. M., & Uzal, F. A. (2018). Identification of Neospora caninum in aborted bovine fetuses of Southern Chile. Brazilian Journal of Veterinary Pathology, 11(2), 37-41. https://doi.org/10.24070/bjvp.1983-0246.v11i2p37-41