Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components

Slobodan Vukicevic, Hynda K. Kleinman, Frank P. Luyten, Anita B. Roberts, Nanette S. Roche, A Hari Reddi

Research output: Contribution to journalArticle

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Abstract

We have recently demonstrated the formation of interconnecting canalicular cell processes in bone cells upon contact with basement membrane components. Here we have determined whether growth factors in the reconstituted basement membrane (Matrigel) were active in influencing the cellular network formation. Various growth factors including transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1, bovine fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were identified in Matrigel. Exogenous TGF-β blocked the cellular network formation. Conversely, addition of TGF-β1 neutralizing antibodies to Matrigel stimulated the cellular network formation. bFGF, EGF, and PDGF all promoted cellular migration and organization on Matrigel. Addition of bFGF to MC3T3-E1 cells grown on Matrigel overcame the inhibitory effect of TGF-β. Some TGF-β remained bound to type IV collagen purified from the Engelbreth-Holm-Swarm tumor matrix. These data demonstrate that reconstituted basement membrane contains growth factors which influence cellular behavior, suggesting caution in the interpretation of experiments on cellular activity related to Matrigel, collagen type IV, and possibly other extracellular matrix components.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalExperimental Cell Research
Volume202
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Basement Membrane
Transforming Growth Factors
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Collagen Type IV
Fibroblast Growth Factors
Platelet-Derived Growth Factor
Epidermal Growth Factor
Fibroblast Growth Factor 1
Somatomedins
Neutralizing Antibodies
matrigel
Bone and Bones
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. / Vukicevic, Slobodan; Kleinman, Hynda K.; Luyten, Frank P.; Roberts, Anita B.; Roche, Nanette S.; Reddi, A Hari.

In: Experimental Cell Research, Vol. 202, No. 1, 1992, p. 1-8.

Research output: Contribution to journalArticle

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