TY - JOUR
T1 - Identification of mosquito bloodmeals collected in diverse habitats in Malaysian borneo using COI barcoding
AU - Young, Katherine I.
AU - Medwid, Joseph T.
AU - Azar, Sasha R.
AU - Huff, Robert M.
AU - Drumm, Hannah
AU - Coffey, Lark L.
AU - Jason Pitts, R.
AU - Buenemann, Michaela
AU - Vasilakis, Nikos
AU - Perera, David
AU - Hanley, Kathryn A.
PY - 2020/6
Y1 - 2020/6
N2 - Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged Aedes albopictus, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). Culex tritaeniohynchus (n = 2), Cx. gelidus (n = 3), and Cx. quiquefasciatus (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito–host networks in a biodiversity hotspot.
AB - Land cover and land use change (LCLUC) acts as a catalyst for spillover of arthropod-borne pathogens into novel hosts by shifting host and vector diversity, abundance, and distribution, ultimately reshaping host–vector interactions. Identification of bloodmeals from wild-caught mosquitoes provides insight into host utilization of particular species in particular land cover types, and hence their potential role in pathogen maintenance and spillover. Here, we collected 134 blood-engorged mosquitoes comprising 10 taxa across 9 land cover types in Sarawak, Malaysian Borneo, a region experiencing intense LCLUC and concomitant spillover of arthropod-borne pathogens. Host sources of blood were successfully identified for 116 (87%) mosquitoes using cytochrome oxidase subunit I (COI) barcoding. A diverse range of hosts were identified, including reptiles, amphibians, birds, and mammals. Sixteen engorged Aedes albopictus, a major vector of dengue virus, were collected from seven land cover types and found to feed exclusively on humans (73%) and boar (27%). Culex tritaeniohynchus (n = 2), Cx. gelidus (n = 3), and Cx. quiquefasciatus (n = 3), vectors of Japanese encephalitis virus, fed on humans and pigs in the rural built-up land cover, creating potential transmission networks between these species. Our data support the use of COI barcoding to characterize mosquito–host networks in a biodiversity hotspot.
KW - Aedes
KW - Arbovirus
KW - Bloodmeal
KW - Borneo
KW - Dengue virus
KW - Host
KW - Land cover and land use change
KW - Mosquito
KW - Vector
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U2 - 10.3390/tropicalmed5020051
DO - 10.3390/tropicalmed5020051
M3 - Article
AN - SCOPUS:85083295784
VL - 5
JO - Tropical Medicine and Infectious Disease
JF - Tropical Medicine and Infectious Disease
SN - 2414-6366
IS - 2
M1 - 51
ER -