Identification of katanin, an ATPase that severs and disassembles stable microtubules

Francis J. McNally, Ronald D. Vale

Research output: Contribution to journalArticle

352 Citations (Scopus)

Abstract

Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.

Original languageEnglish (US)
Pages (from-to)419-429
Number of pages11
JournalCell
Volume75
Issue number3
DOIs
StatePublished - Nov 5 1993

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Tubulin
Microtubules
Adenosine Triphosphatases
Hydrolysis
Dimers
Polymers
Nucleotides
Adenosine Triphosphate
Cells
Eukaryotic Cells
Cytoskeleton
Cell Movement
katanin
Cell Differentiation
Cell Cycle
Enzymes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Identification of katanin, an ATPase that severs and disassembles stable microtubules. / McNally, Francis J.; Vale, Ronald D.

In: Cell, Vol. 75, No. 3, 05.11.1993, p. 419-429.

Research output: Contribution to journalArticle

McNally, Francis J. ; Vale, Ronald D. / Identification of katanin, an ATPase that severs and disassembles stable microtubules. In: Cell. 1993 ; Vol. 75, No. 3. pp. 419-429.
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