Abstract
Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 419-429 |
| Number of pages | 11 |
| Journal | Cell |
| Volume | 75 |
| Issue number | 3 |
| DOIs | |
| State | Published - Nov 5 1993 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Identification of katanin, an ATPase that severs and disassembles stable microtubules. / McNally, Francis J.; Vale, Ronald D.
In: Cell, Vol. 75, No. 3, 05.11.1993, p. 419-429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of katanin, an ATPase that severs and disassembles stable microtubules
AU - McNally, Francis J.
AU - Vale, Ronald D.
PY - 1993/11/5
Y1 - 1993/11/5
N2 - Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.
AB - Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027424297&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(93)90377-3
DO - 10.1016/0092-8674(93)90377-3
M3 - Article
C2 - 8221885
AN - SCOPUS:0027424297
VL - 75
SP - 419
EP - 429
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -