TY - JOUR
T1 - Identification of katanin, an ATPase that severs and disassembles stable microtubules
AU - McNally, Francis J.
AU - Vale, Ronald D.
PY - 1993/11/5
Y1 - 1993/11/5
N2 - Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.
AB - Eukaryotic cells rapidly reorganize their microtubule cytoskeleton during the cell cycle, differentiation, and cell migration. In this study, we have purified a heterodimeric protein, katanin, that severs and disassembles microtubules to tubulin dimers. The disassembled tubulin can repolymerize, indicating that it is not irreversibly modified or denatured in the reaction. Katanin is a microtubule-stimulated ATPase and requires ATP hydrolysis to sever microtubules. Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells.
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U2 - 10.1016/0092-8674(93)90377-3
DO - 10.1016/0092-8674(93)90377-3
M3 - Article
C2 - 8221885
AN - SCOPUS:0027424297
VL - 75
SP - 419
EP - 429
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -