Abstract
Serum proteomics analysis may lead to the discovery of novel osteoporosis biomarkers. The Osteoporotic Fractures in Men (MrOS) study comprises men ≥65 years old in the US who have had repeated BMD measures and have been followed for incident fracture. High-throughput quantitative proteomic analysis was performed on baseline fasting serum samples from non-Hispanic white men using a multidimensional approach coupling liquid chromatography, ion-mobility separation, and mass spectrometry (LC-IMS-MS). We followed the participants for a mean of 4.6 years for changes in femoral neck bone mineral density (BMD) and for incident hip fracture. Change in BMD was determined from mixed effects regression models taking age and weight into account. Participants were categorized into three groups: BMD maintenance (no decline; estimated change ≥0g/cm2, n=453); expected loss (estimated change 0 to 1 SD below the estimated mean change, -0.034g/cm2 for femoral neck, n=1184); and accelerated loss (estimated change ≥1 SD below mean change, n=237). Differential abundance values of 3946 peptides were summarized by meta-analysis to determine differential abundance of each of 339 corresponding proteins for accelerated BMD loss versus maintenance. Using this meta-analytic standardized fold change at cutoffs of ≥1.1 or ≤0.9 (p<0.10), 20 proteins were associated with accelerated BMD loss. Associations of those 20 proteins with incident hip fracture were tested using Cox proportional hazards models with age and BMI adjustment in 2473 men. Five proteins were associated with incident hip fracture (HR between 1.29 and 1.41 per SD increase in estimated protein abundance). Some proteins have been previously associated with fracture risk (eg, CD14 and SHBG), whereas others have roles in cellular senescence and aging (B2MG and TIMP1) and complement activation and innate immunity (CO7, CO9, CFAD). These findings may inform development of biomarkers for future research in bone biology and fracture prediction.
| Original language | English (US) |
|---|---|
| Journal | Journal of Bone and Mineral Research |
| DOIs | |
| State | Accepted/In press - 2017 |
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Keywords
- Biochemical markers of bone turnover
- General population studies
- Osteoporosis
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Orthopedics and Sports Medicine
Cite this
Identification of Hip BMD Loss and Fracture Risk Markers Through Population-Based Serum Proteomics. / Nielson, Carrie M.; Wiedrick, Jack; Shen, Jian; Jacobs, Jon; Baker, Erin S.; Baraff, Aaron; Piehowski, Paul; Lee, Christine G.; Baratt, Arie; Petyuk, Vladislav; Mcweeney, Shannon; Lim, Jeong Youn; Bauer, Douglas C.; Lane, Nancy E; Cawthon, Peggy M.; Smith, Richard D.; Lapidus, Jodi; Orwoll, Eric S.
In: Journal of Bone and Mineral Research, 2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of Hip BMD Loss and Fracture Risk Markers Through Population-Based Serum Proteomics
AU - Nielson, Carrie M.
AU - Wiedrick, Jack
AU - Shen, Jian
AU - Jacobs, Jon
AU - Baker, Erin S.
AU - Baraff, Aaron
AU - Piehowski, Paul
AU - Lee, Christine G.
AU - Baratt, Arie
AU - Petyuk, Vladislav
AU - Mcweeney, Shannon
AU - Lim, Jeong Youn
AU - Bauer, Douglas C.
AU - Lane, Nancy E
AU - Cawthon, Peggy M.
AU - Smith, Richard D.
AU - Lapidus, Jodi
AU - Orwoll, Eric S.
PY - 2017
Y1 - 2017
N2 - Serum proteomics analysis may lead to the discovery of novel osteoporosis biomarkers. The Osteoporotic Fractures in Men (MrOS) study comprises men ≥65 years old in the US who have had repeated BMD measures and have been followed for incident fracture. High-throughput quantitative proteomic analysis was performed on baseline fasting serum samples from non-Hispanic white men using a multidimensional approach coupling liquid chromatography, ion-mobility separation, and mass spectrometry (LC-IMS-MS). We followed the participants for a mean of 4.6 years for changes in femoral neck bone mineral density (BMD) and for incident hip fracture. Change in BMD was determined from mixed effects regression models taking age and weight into account. Participants were categorized into three groups: BMD maintenance (no decline; estimated change ≥0g/cm2, n=453); expected loss (estimated change 0 to 1 SD below the estimated mean change, -0.034g/cm2 for femoral neck, n=1184); and accelerated loss (estimated change ≥1 SD below mean change, n=237). Differential abundance values of 3946 peptides were summarized by meta-analysis to determine differential abundance of each of 339 corresponding proteins for accelerated BMD loss versus maintenance. Using this meta-analytic standardized fold change at cutoffs of ≥1.1 or ≤0.9 (p<0.10), 20 proteins were associated with accelerated BMD loss. Associations of those 20 proteins with incident hip fracture were tested using Cox proportional hazards models with age and BMI adjustment in 2473 men. Five proteins were associated with incident hip fracture (HR between 1.29 and 1.41 per SD increase in estimated protein abundance). Some proteins have been previously associated with fracture risk (eg, CD14 and SHBG), whereas others have roles in cellular senescence and aging (B2MG and TIMP1) and complement activation and innate immunity (CO7, CO9, CFAD). These findings may inform development of biomarkers for future research in bone biology and fracture prediction.
AB - Serum proteomics analysis may lead to the discovery of novel osteoporosis biomarkers. The Osteoporotic Fractures in Men (MrOS) study comprises men ≥65 years old in the US who have had repeated BMD measures and have been followed for incident fracture. High-throughput quantitative proteomic analysis was performed on baseline fasting serum samples from non-Hispanic white men using a multidimensional approach coupling liquid chromatography, ion-mobility separation, and mass spectrometry (LC-IMS-MS). We followed the participants for a mean of 4.6 years for changes in femoral neck bone mineral density (BMD) and for incident hip fracture. Change in BMD was determined from mixed effects regression models taking age and weight into account. Participants were categorized into three groups: BMD maintenance (no decline; estimated change ≥0g/cm2, n=453); expected loss (estimated change 0 to 1 SD below the estimated mean change, -0.034g/cm2 for femoral neck, n=1184); and accelerated loss (estimated change ≥1 SD below mean change, n=237). Differential abundance values of 3946 peptides were summarized by meta-analysis to determine differential abundance of each of 339 corresponding proteins for accelerated BMD loss versus maintenance. Using this meta-analytic standardized fold change at cutoffs of ≥1.1 or ≤0.9 (p<0.10), 20 proteins were associated with accelerated BMD loss. Associations of those 20 proteins with incident hip fracture were tested using Cox proportional hazards models with age and BMI adjustment in 2473 men. Five proteins were associated with incident hip fracture (HR between 1.29 and 1.41 per SD increase in estimated protein abundance). Some proteins have been previously associated with fracture risk (eg, CD14 and SHBG), whereas others have roles in cellular senescence and aging (B2MG and TIMP1) and complement activation and innate immunity (CO7, CO9, CFAD). These findings may inform development of biomarkers for future research in bone biology and fracture prediction.
KW - Biochemical markers of bone turnover
KW - General population studies
KW - Osteoporosis
UR - http://www.scopus.com/inward/record.url?scp=85017431107&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017431107&partnerID=8YFLogxK
U2 - 10.1002/jbmr.3125
DO - 10.1002/jbmr.3125
M3 - Article
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
SN - 0884-0431
ER -