Identification of germ-line stem cells in Zebrafish

Bruce W. Draper

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages103-113
Number of pages11
Volume1463
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Fingerprint

Zebrafish
Germ Cells
Stem Cells
Gonads
Fluorescence In Situ Hybridization
Fluorescent Antibody Technique
Testis
Ovary
Fishes
RNA
Messenger RNA
Population
Genes

Keywords

  • Confocal immunofluorescence
  • Fluorescent RNA in situ hybridization
  • Nanos2
  • Oogonial stem cell
  • Spermatogonial stem cell
  • Vasa
  • Zebrafish germ-line stem cells
  • Zebrafish ovary
  • Zebrafish testis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Draper, B. W. (2017). Identification of germ-line stem cells in Zebrafish. In Methods in Molecular Biology (Vol. 1463, pp. 103-113). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_8

Identification of germ-line stem cells in Zebrafish. / Draper, Bruce W.

Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. p. 103-113 (Methods in Molecular Biology; Vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Draper, BW 2017, Identification of germ-line stem cells in Zebrafish. in Methods in Molecular Biology. vol. 1463, Methods in Molecular Biology, vol. 1463, Humana Press Inc., pp. 103-113. https://doi.org/10.1007/978-1-4939-4017-2_8
Draper BW. Identification of germ-line stem cells in Zebrafish. In Methods in Molecular Biology. Vol. 1463. Humana Press Inc. 2017. p. 103-113. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-4017-2_8
Draper, Bruce W. / Identification of germ-line stem cells in Zebrafish. Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. pp. 103-113 (Methods in Molecular Biology).
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