Abstract
The natural porosity of eggshells allows hen eggs to become contaminated with microbes from the nesting material and environment. Those microorganisms can later proliferate due to the humid ambient conditions while stored in refrigerators, causing a potential health hazard to the consumer. The microbes’ volatile organic compounds (mVOCs) are released by both fungi and bacteria. We studied mVOCs produced by aging eggs likely contaminated by fungi and fresh eggs using the non-invasive detection method of gas-phase sampling of volatiles followed by gas chromatography/mass spectrometry (GC/MS) analysis. Two different fungal species (Cladosporium macrocarpum and Botrytis cinerea) and two different bacteria species (Stenotrophomas rhizophila and Pseudomonas argentinensis) were identified inside the studied eggs. Two compounds believed to originate from the fungi themselves were identified. One fungus-specific compound was found in both egg and the fungi: trichloromethane. [Figure not available: see fulltext.]
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6649-6658 |
| Number of pages | 10 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 408 |
| Issue number | 24 |
| DOIs | |
| State | Published - Sep 1 2016 |
Fingerprint
Keywords
- Bacteria
- Fungi
- Gas chromatography/mass spectrometry (GC/MS)
- Hen egg
- Solid-phase microextraction (SPME)
- Volatile organic compounds (VOCs)
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Medicine(all)
Cite this
Identification of fungal metabolites from inside Gallus gallus domesticus eggshells by non-invasively detecting volatile organic compounds (VOCs). / Cumeras, Raquel; Aksenov, Alexander A.; Pasamontes, Alberto; Fung, Alexander G.; Cianchetta, Amanda N.; Doan, Hung; Davis, R. Michael; Davis, Cristina E.
In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 24, 01.09.2016, p. 6649-6658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of fungal metabolites from inside Gallus gallus domesticus eggshells by non-invasively detecting volatile organic compounds (VOCs)
AU - Cumeras, Raquel
AU - Aksenov, Alexander A.
AU - Pasamontes, Alberto
AU - Fung, Alexander G.
AU - Cianchetta, Amanda N.
AU - Doan, Hung
AU - Davis, R. Michael
AU - Davis, Cristina E
PY - 2016/9/1
Y1 - 2016/9/1
N2 - The natural porosity of eggshells allows hen eggs to become contaminated with microbes from the nesting material and environment. Those microorganisms can later proliferate due to the humid ambient conditions while stored in refrigerators, causing a potential health hazard to the consumer. The microbes’ volatile organic compounds (mVOCs) are released by both fungi and bacteria. We studied mVOCs produced by aging eggs likely contaminated by fungi and fresh eggs using the non-invasive detection method of gas-phase sampling of volatiles followed by gas chromatography/mass spectrometry (GC/MS) analysis. Two different fungal species (Cladosporium macrocarpum and Botrytis cinerea) and two different bacteria species (Stenotrophomas rhizophila and Pseudomonas argentinensis) were identified inside the studied eggs. Two compounds believed to originate from the fungi themselves were identified. One fungus-specific compound was found in both egg and the fungi: trichloromethane. [Figure not available: see fulltext.]
AB - The natural porosity of eggshells allows hen eggs to become contaminated with microbes from the nesting material and environment. Those microorganisms can later proliferate due to the humid ambient conditions while stored in refrigerators, causing a potential health hazard to the consumer. The microbes’ volatile organic compounds (mVOCs) are released by both fungi and bacteria. We studied mVOCs produced by aging eggs likely contaminated by fungi and fresh eggs using the non-invasive detection method of gas-phase sampling of volatiles followed by gas chromatography/mass spectrometry (GC/MS) analysis. Two different fungal species (Cladosporium macrocarpum and Botrytis cinerea) and two different bacteria species (Stenotrophomas rhizophila and Pseudomonas argentinensis) were identified inside the studied eggs. Two compounds believed to originate from the fungi themselves were identified. One fungus-specific compound was found in both egg and the fungi: trichloromethane. [Figure not available: see fulltext.]
KW - Bacteria
KW - Fungi
KW - Gas chromatography/mass spectrometry (GC/MS)
KW - Hen egg
KW - Solid-phase microextraction (SPME)
KW - Volatile organic compounds (VOCs)
UR - http://www.scopus.com/inward/record.url?scp=84986211360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986211360&partnerID=8YFLogxK
U2 - 10.1007/s00216-016-9778-3
DO - 10.1007/s00216-016-9778-3
M3 - Article
VL - 408
SP - 6649
EP - 6658
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 24
ER -