Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis

Sara E. Miller, Richard M Levenson, Claire Aldridge, Susan Hester, Daniel J. Kenan, David N. Howell

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.

Original languageEnglish (US)
Pages (from-to)183-193
Number of pages11
JournalUltrastructural Pathology
Volume21
Issue number2
StatePublished - 1997
Externally publishedYes

Keywords

  • Confocal microscopy
  • Electron microscopy
  • Pathology
  • Virus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Instrumentation
  • Structural Biology

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