Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno County, California

Rory D. McAbee, Emily N. Green, Jodie Holeman, Julie Christiansen, Niki Frye, Katherine Dealey, F. Steve Mulligan, Aaron Brault, Anthony J. Cornel

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Culex pipiens sensu lato mosquitoes were collected from 24 gravid traps (mid-June to mid-October, 2005) in Fresno County, CA. Captured gravid females were allowed to oviposit before sibling species identification by Ace.2 PCR and detection of West Nile virus (WNV) RNA by RT-PCR were performed on the mother and her offspring. Of the 442 Cx. pipiens s.l. female mosquitoes collected, 88 were positive for WNV viral RNA (peaked in August) with no significant differences among complex members or habitat. Vertical transmission was detected in 4 out of 20 families originating from WNV-positive mothers, however, in only a small number of offspring from each family. Out of 101 families that had PCR-based maternal and offspring identifications, the offspring from 15 families produced inexplicable amplicon patterns, suggesting ambiguities in the PCR assay identifications. Male genitalia (DV/D ratio) and Ace.2 PCR identifications revealed numerous discrepancies in our ability to accurately determine the identity of Cx. pipiens complex members in the hybrid zone of Fresno County.

Original languageEnglish (US)
Pages (from-to)303-310
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume78
Issue number2
StatePublished - Feb 1 2008

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West Nile virus
Culex
Culicidae
Polymerase Chain Reaction
Male Genitalia
Viral RNA
Ecosystem
Mothers
RNA

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

McAbee, R. D., Green, E. N., Holeman, J., Christiansen, J., Frye, N., Dealey, K., ... Cornel, A. J. (2008). Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno County, California. American Journal of Tropical Medicine and Hygiene, 78(2), 303-310.

Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno County, California. / McAbee, Rory D.; Green, Emily N.; Holeman, Jodie; Christiansen, Julie; Frye, Niki; Dealey, Katherine; Mulligan, F. Steve; Brault, Aaron; Cornel, Anthony J.

In: American Journal of Tropical Medicine and Hygiene, Vol. 78, No. 2, 01.02.2008, p. 303-310.

Research output: Contribution to journalArticle

McAbee, RD, Green, EN, Holeman, J, Christiansen, J, Frye, N, Dealey, K, Mulligan, FS, Brault, A & Cornel, AJ 2008, 'Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno County, California', American Journal of Tropical Medicine and Hygiene, vol. 78, no. 2, pp. 303-310.
McAbee, Rory D. ; Green, Emily N. ; Holeman, Jodie ; Christiansen, Julie ; Frye, Niki ; Dealey, Katherine ; Mulligan, F. Steve ; Brault, Aaron ; Cornel, Anthony J. / Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno County, California. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 78, No. 2. pp. 303-310.
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