Identification of chromatin-related protein interactions using protein microarrays

Matthew A Coleman, Kristi A. Miller, Peter T. Beernink, Daniel M. Yoshikawa, Joanna S. Albala

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Dynamic structural changes in chromatin are mediated by protein interactions that modulate multiple cellular processes including replication, transcription, recombination and DNA repair. Complexes that recognize chromatin are defined by several distinct groups of proteins that either directly modify histones or interact with histone-DNA complexes. A protein microarray format was used to analyze the interaction of various DNA repair proteins with chromatin components. We applied proteins, antibodies and DNA to functionalized glass slides and interrogated the slides with our proteins of interest to identify novel protein-protein interactions for proteins involved in DNA double-strand break repair. Here we demonstrate that the DNA repair protein RAD51B, and not its cognate partner RAD51C, interacts with histones and not nucleosomes. Nucleosome-specific interactions were demonstrated with the recently identified SWI/SNF protein, SMARCAL1. Unique RAD51B-histone interactions were corroborated using Far Western analysis. This is the first demonstration of an interaction between RAD51B and histone proteins that may be important for the successful repair of DNA double-strand breaks.

Original languageEnglish (US)
Pages (from-to)2101-2107
Number of pages7
JournalProteomics
Volume3
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Keywords

  • Histone
  • Nucleosome
  • Protein array
  • RAD51B
  • RAD51C
  • SMARCAL1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Coleman, M. A., Miller, K. A., Beernink, P. T., Yoshikawa, D. M., & Albala, J. S. (2003). Identification of chromatin-related protein interactions using protein microarrays. Proteomics, 3(11), 2101-2107. https://doi.org/10.1002/pmic.200300593