Identification of catalase-negative, non-beta-hemolytic, gram-positive cocci isolated from milk samples

Madeleine Fortin, Serge Messier, Julie Paré, Robert Higgins

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

This study was undertaken in an effort to improve the identification scheme of catalase-negative, non-betahemolytic, gram-positive cocci isolated from milk samples obtained from cows. First, the sensitivity and specificity of the identification procedure currently in use in our laboratory were compared to the results obtained with API 20 STREP strips which were set as the gold standard. Second, a number of other identification tests, which could contribute to increase the sensitivity and specificity of the identification procedure of these microorganisms, were evaluated and selected. The data have shown that there is a necessity to review the identification procedure. Some modifications are suggested to laboratories doing milk sample analyses. A standardized procedure, using the CAMP test, esculin and sodium hippurate hydrolysis, the presence of the enzymes pyrolidonyl arylaminase and leucine aminopeptidase, and acid production from 1% inulin and raffinose broth, would not only improve the results of the identification process of gram-positive cocci isolated from milk samples but also ensure greater uniformity of the epidemiological data.

Original languageEnglish (US)
Pages (from-to)106-109
Number of pages4
JournalJournal of Clinical Microbiology
Volume41
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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