Identification of bluetongue virus protein-specific antibody responses in sheep by immunoblotting.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A western immunoblotting procedure was developed for identifying bluetongue virus protein-specific antibody responses in sheep. Assay conditions were optimized and included electrophoretic transfer of viral proteins to a nitrocellulose membrane (NCM), blocking of unbound sites on the NCM, and detection of NCM-bound primary immune complexes. A biotin-avidin-enzyme system was determined to be superior in terms of sensitivity for identification of the NCM-bound virus protein-antibody complexes. The biotin-avidin-enzyme detection system made use of biotinylated rabbit anti-sheep IgG and horseradish peroxidase-conjugated egg white avidin. This system permitted identification of antibodies specific for up to 10 bluetongue virus-associated proteins, 4 of which have not been characterized.

Original languageEnglish (US)
Pages (from-to)1194-1198
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume48
Issue number8
StatePublished - Aug 1987

Fingerprint

Bluetongue virus
avidin
Collodion
immunoblotting
Immunoblotting
Antibody Formation
Avidin
Sheep
biotin
sheep
antibodies
Membranes
Biotin
antigen-antibody complex
Proteins
proteins
viral proteins
egg albumen
enzymes
Egg White

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Identification of bluetongue virus protein-specific antibody responses in sheep by immunoblotting. / Adkison, M. A.; Stott, Jeffrey L; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 48, No. 8, 08.1987, p. 1194-1198.

Research output: Contribution to journalArticle

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