Abstract
A western immunoblotting procedure was developed for identifying bluetongue virus protein-specific antibody responses in sheep. Assay conditions were optimized and included electrophoretic transfer of viral proteins to a nitrocellulose membrane (NCM), blocking of unbound sites on the NCM, and detection of NCM-bound primary immune complexes. A biotin-avidin-enzyme system was determined to be superior in terms of sensitivity for identification of the NCM-bound virus protein-antibody complexes. The biotin-avidin-enzyme detection system made use of biotinylated rabbit anti-sheep IgG and horseradish peroxidase-conjugated egg white avidin. This system permitted identification of antibodies specific for up to 10 bluetongue virus-associated proteins, 4 of which have not been characterized.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1194-1198 |
| Number of pages | 5 |
| Journal | American Journal of Veterinary Research |
| Volume | 48 |
| Issue number | 8 |
| State | Published - Aug 1987 |
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- veterinary(all)
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Identification of bluetongue virus protein-specific antibody responses in sheep by immunoblotting. / Adkison, M. A.; Stott, Jeffrey L; Osburn, Bennie.
In: American Journal of Veterinary Research, Vol. 48, No. 8, 08.1987, p. 1194-1198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of bluetongue virus protein-specific antibody responses in sheep by immunoblotting.
AU - Adkison, M. A.
AU - Stott, Jeffrey L
AU - Osburn, Bennie
PY - 1987/8
Y1 - 1987/8
N2 - A western immunoblotting procedure was developed for identifying bluetongue virus protein-specific antibody responses in sheep. Assay conditions were optimized and included electrophoretic transfer of viral proteins to a nitrocellulose membrane (NCM), blocking of unbound sites on the NCM, and detection of NCM-bound primary immune complexes. A biotin-avidin-enzyme system was determined to be superior in terms of sensitivity for identification of the NCM-bound virus protein-antibody complexes. The biotin-avidin-enzyme detection system made use of biotinylated rabbit anti-sheep IgG and horseradish peroxidase-conjugated egg white avidin. This system permitted identification of antibodies specific for up to 10 bluetongue virus-associated proteins, 4 of which have not been characterized.
AB - A western immunoblotting procedure was developed for identifying bluetongue virus protein-specific antibody responses in sheep. Assay conditions were optimized and included electrophoretic transfer of viral proteins to a nitrocellulose membrane (NCM), blocking of unbound sites on the NCM, and detection of NCM-bound primary immune complexes. A biotin-avidin-enzyme system was determined to be superior in terms of sensitivity for identification of the NCM-bound virus protein-antibody complexes. The biotin-avidin-enzyme detection system made use of biotinylated rabbit anti-sheep IgG and horseradish peroxidase-conjugated egg white avidin. This system permitted identification of antibodies specific for up to 10 bluetongue virus-associated proteins, 4 of which have not been characterized.
UR - http://www.scopus.com/inward/record.url?scp=0023395801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023395801&partnerID=8YFLogxK
M3 - Article
C2 - 2820278
AN - SCOPUS:0023395801
VL - 48
SP - 1194
EP - 1198
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 8
ER -