Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov.

Frederique B. Schulte Fischedick, Matthew J. Stuckey, Alvaro Aguilar-Setién, Hayde Moreno-Sandoval, Guillermo Galvez-Romero, Mónica Salas-Rojas, Nidia Arechiga-Ceballos, Paul A M Overgaauw, Rickie W. Kasten, Bruno B Chomel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.

Original languageEnglish (US)
Pages (from-to)636-642
Number of pages7
JournalVector-Borne and Zoonotic Diseases
Volume16
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

Bartonella
Mexico
Rodentia
Peromyscus
Thrombosis
Essential Genes
Bacillus
Names
Genotype
Polymerase Chain Reaction
DNA
Genes

Keywords

  • Bartonella vinsonii subsp arupensis
  • Bartonella vinsonii subsp. vinsonii
  • Mexico
  • Peromyscus yucatanicus
  • Yucatan

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

Cite this

Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov. / Schulte Fischedick, Frederique B.; Stuckey, Matthew J.; Aguilar-Setién, Alvaro; Moreno-Sandoval, Hayde; Galvez-Romero, Guillermo; Salas-Rojas, Mónica; Arechiga-Ceballos, Nidia; Overgaauw, Paul A M; Kasten, Rickie W.; Chomel, Bruno B.

In: Vector-Borne and Zoonotic Diseases, Vol. 16, No. 10, 01.10.2016, p. 636-642.

Research output: Contribution to journalArticle

Schulte Fischedick, FB, Stuckey, MJ, Aguilar-Setién, A, Moreno-Sandoval, H, Galvez-Romero, G, Salas-Rojas, M, Arechiga-Ceballos, N, Overgaauw, PAM, Kasten, RW & Chomel, BB 2016, 'Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov.', Vector-Borne and Zoonotic Diseases, vol. 16, no. 10, pp. 636-642. https://doi.org/10.1089/vbz.2016.1981
Schulte Fischedick, Frederique B. ; Stuckey, Matthew J. ; Aguilar-Setién, Alvaro ; Moreno-Sandoval, Hayde ; Galvez-Romero, Guillermo ; Salas-Rojas, Mónica ; Arechiga-Ceballos, Nidia ; Overgaauw, Paul A M ; Kasten, Rickie W. ; Chomel, Bruno B. / Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov. In: Vector-Borne and Zoonotic Diseases. 2016 ; Vol. 16, No. 10. pp. 636-642.
@article{ea50e7fdb6264758abfe498a3687cac9,
title = "Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov.",
abstract = "Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58{\%}) rodents. All Bartonella-positive rodents were Yucat{\'a}n deer mice from San Jos{\'e} Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.",
keywords = "Bartonella vinsonii subsp arupensis, Bartonella vinsonii subsp. vinsonii, Mexico, Peromyscus yucatanicus, Yucatan",
author = "{Schulte Fischedick}, {Frederique B.} and Stuckey, {Matthew J.} and Alvaro Aguilar-Seti{\'e}n and Hayde Moreno-Sandoval and Guillermo Galvez-Romero and M{\'o}nica Salas-Rojas and Nidia Arechiga-Ceballos and Overgaauw, {Paul A M} and Kasten, {Rickie W.} and Chomel, {Bruno B}",
year = "2016",
month = "10",
day = "1",
doi = "10.1089/vbz.2016.1981",
language = "English (US)",
volume = "16",
pages = "636--642",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert Inc.",
number = "10",

}

TY - JOUR

T1 - Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov.

AU - Schulte Fischedick, Frederique B.

AU - Stuckey, Matthew J.

AU - Aguilar-Setién, Alvaro

AU - Moreno-Sandoval, Hayde

AU - Galvez-Romero, Guillermo

AU - Salas-Rojas, Mónica

AU - Arechiga-Ceballos, Nidia

AU - Overgaauw, Paul A M

AU - Kasten, Rickie W.

AU - Chomel, Bruno B

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.

AB - Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.

KW - Bartonella vinsonii subsp arupensis

KW - Bartonella vinsonii subsp. vinsonii

KW - Mexico

KW - Peromyscus yucatanicus

KW - Yucatan

UR - http://www.scopus.com/inward/record.url?scp=84989897891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989897891&partnerID=8YFLogxK

U2 - 10.1089/vbz.2016.1981

DO - 10.1089/vbz.2016.1981

M3 - Article

C2 - 27626126

AN - SCOPUS:84989897891

VL - 16

SP - 636

EP - 642

JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 10

ER -