Abstract
Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 636-642 |
| Number of pages | 7 |
| Journal | Vector-Borne and Zoonotic Diseases |
| Volume | 16 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2016 |
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Keywords
- Bartonella vinsonii subsp arupensis
- Bartonella vinsonii subsp. vinsonii
- Mexico
- Peromyscus yucatanicus
- Yucatan
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
- Virology
Cite this
Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov. / Schulte Fischedick, Frederique B.; Stuckey, Matthew J.; Aguilar-Setién, Alvaro; Moreno-Sandoval, Hayde; Galvez-Romero, Guillermo; Salas-Rojas, Mónica; Arechiga-Ceballos, Nidia; Overgaauw, Paul A M; Kasten, Rickie W.; Chomel, Bruno B.
In: Vector-Borne and Zoonotic Diseases, Vol. 16, No. 10, 01.10.2016, p. 636-642.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of Bartonella Species Isolated from Rodents from Yucatan, Mexico, and Isolation of Bartonella vinsonii subsp. yucatanensis subsp. nov.
AU - Schulte Fischedick, Frederique B.
AU - Stuckey, Matthew J.
AU - Aguilar-Setién, Alvaro
AU - Moreno-Sandoval, Hayde
AU - Galvez-Romero, Guillermo
AU - Salas-Rojas, Mónica
AU - Arechiga-Ceballos, Nidia
AU - Overgaauw, Paul A M
AU - Kasten, Rickie W.
AU - Chomel, Bruno B
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.
AB - Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents. Overall, Bartonella spp. were isolated from the blood/blood clots of 22 (58%) rodents. All Bartonella-positive rodents were Yucatán deer mice from San José Pituch. Sequencing of a fragment of the gltA gene showed that all but one rodent isolates were closest to B. vinsonii subsp. vinsonii and one isolate was intermediate between B. vinsonii subsp. berkhoffii and B. vinsonii subsp. arupensis. Further analysis of concatenated housekeeping genes (gltA, ftsZ, rpoB, and 16S rRNA) suggests that this outlier isolate is a new subspecies within the B. vinsonii genogroup, for which we proposed the name B. vinsonii subsp. yucatanensis.
KW - Bartonella vinsonii subsp arupensis
KW - Bartonella vinsonii subsp. vinsonii
KW - Mexico
KW - Peromyscus yucatanicus
KW - Yucatan
UR - http://www.scopus.com/inward/record.url?scp=84989897891&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84989897891&partnerID=8YFLogxK
U2 - 10.1089/vbz.2016.1981
DO - 10.1089/vbz.2016.1981
M3 - Article
C2 - 27626126
AN - SCOPUS:84989897891
VL - 16
SP - 636
EP - 642
JO - Vector-Borne and Zoonotic Diseases
JF - Vector-Borne and Zoonotic Diseases
SN - 1530-3667
IS - 10
ER -