Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics

Yong Seung Shin, Eung Goo Lee, Gee Wook Shin, Young Rim Kim, Eun Young Lee, Jae Hoon Kim, Hwan Jang, Laurel J Gershwin, Dae Yong Kim, Yong Hwan Kim, Gon Sup Kim, Myung Deuk Suh, Tae Sung Jung

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Antigenic proteins of Neospora caninum (N. caninum) against bovine immunoglobulins M, E, A, and G were investigated by using immunoproteomics. Proteins of N. caninum (KBA-2) tachyzoite lysates separated by two-dimensional gel electrophoresis were transferred to polyvinylidene difluoride (PVDF) membranes, probed with different bovine immunoglobulin class and classified. Antigenic spots recognized were also identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis. 132, 84, 4, and 40 antigenic protein spots were recognized on N. caninum immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. Of these protein spots, the antigenic proteins recognized by either IgM, IgE, and IgG, or IgM and IgG were HSP70, pyruvate kinase, actin, NCDG-1, tubulin α-chain, and putative ribosomal protein S2. On the other hand, IgM, IgE, and IgA reacted with NTPase, HSP60, tubulin β-chain, putative protein disulfide isomerase, enolase, lactate dehydrogenase, serine-threonine phosphatase, 14-3-3 protein homologue, and GRA2 protein. Most of the antigenic proteins identified were associated with the process of invasion, proliferation, and egression of apicomplexans. In our study, HSP70, actin, NTPase, HSP60, pyruvate kinase, enolase, putative ribosomal protein S2, NCDG-1, and GRA2 proteins were found to be immunodominant proteins, which may contribute to the development of diagnostic markers and vaccine.

Original languageEnglish (US)
Pages (from-to)3600-3609
Number of pages10
Issue number11
StatePublished - Nov 2004


  • Antigenic protein
  • Bovine immunoglobulin class
  • Immunoproteomics
  • Neospora caninum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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