Identification of an additional neutralization determinant of equine arteritis virus

Jianqiang Zhang, Peter J. Timoney, Nigel J Maclachlan, Udeni B R Balasuriya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We recently established an in vitro model of equine arteritis virus (EAV) persistence in HeLa cells. The objective of this study was to determine whether viral variants with novel neutralization phenotypes emerged during persistent EAV infection of HeLa cells, as occurs during viral persistence in carrier stallions. Viruses recovered from persistently infected HeLa cells had different neutralization phenotypes than the virus in the original inoculum, as determined by neutralization assays using EAV-specific monoclonal antibodies and polyclonal equine antisera raised against different strains of EAV. Comparative sequence analyses of the entire structural protein genes (ORFs 2a, 2b, and 3-7) of these viruses, coupled with construction of chimeric viruses utilizing an infectious cDNA clone of EAV, confirmed that the alterations in neutralization phenotype were caused by amino acid changes in the GP5 protein encoded by ORF5. Site-directed mutagenesis studies unequivocally confirmed that amino acid 98 in the GP5 protein was responsible for the altered neutralization phenotype of these viruses. Amino acid 98 in the GP5 protein, which has not previously been identified as a neutralization determinant of EAV, should be included in an expanded neutralization site D (amino acids 98-106).

Original languageEnglish (US)
Pages (from-to)150-153
Number of pages4
JournalVirus Research
Volume138
Issue number1-2
DOIs
StatePublished - Dec 2008

Fingerprint

Equine Arteritis Virus
Viruses
HeLa Cells
Phenotype
Amino Acids
Proteins
Virus Diseases
Site-Directed Mutagenesis
Open Reading Frames
Horses
Sequence Analysis
Immune Sera
Complementary DNA
Clone Cells
Monoclonal Antibodies

Keywords

  • Equine arteritis virus
  • HeLa cells
  • Neutralization determinant
  • Persistent infection

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Identification of an additional neutralization determinant of equine arteritis virus. / Zhang, Jianqiang; Timoney, Peter J.; Maclachlan, Nigel J; Balasuriya, Udeni B R.

In: Virus Research, Vol. 138, No. 1-2, 12.2008, p. 150-153.

Research output: Contribution to journalArticle

Zhang, Jianqiang ; Timoney, Peter J. ; Maclachlan, Nigel J ; Balasuriya, Udeni B R. / Identification of an additional neutralization determinant of equine arteritis virus. In: Virus Research. 2008 ; Vol. 138, No. 1-2. pp. 150-153.
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