Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS/MS

Fengyun Liao, Aiming Yu, Jianye Yu, Ding Wang, Yinai Wu, Huazhu Zheng, Yingjiao Meng, Dongmei He, Xiao Shen, Lisheng Wang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

BuyangHuanwu decoction (BHD) is widely used as a traditional herbal medicine because of its antithrombotic effect, which is attributed to the inhibition of platelet aggregation; however, its active compounds remain unknown. In this study, we developed a method involving platelet binding, solid-phase extraction, and HPLC-MS/MS for screening BHD compounds with potential anti-platelet aggregation properties. Five compounds showing platelet binding affinity were identified as 6-hydroxykaempferol-di-O-glucoside, paeoniflorin, calycosin-7-O-β-D-glucoside, galloylpaeoniflorin, and formononetin-7-O-β-D-glucoside. The results of anti-platelet aggregation experiments in vitro confirmed that these compounds inhibited adenosine diphosphate-induced platelet aggregation. Our results suggest that a platelet binding assay combined with solid-phase extraction and HPLC-MS/MS is an effective method for screening anti-platelet aggregation agents in traditional Chinese medicines.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1092
DOIs
StatePublished - Aug 15 2018
Externally publishedYes

Keywords

  • Anti-platelet aggregation
  • BuyangHuanwu decoction
  • Platelet binding assay
  • Solid phase extraction
  • Traditional Chinese medicines

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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