TY - JOUR
T1 - Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS/MS
AU - Liao, Fengyun
AU - Yu, Aiming
AU - Yu, Jianye
AU - Wang, Ding
AU - Wu, Yinai
AU - Zheng, Huazhu
AU - Meng, Yingjiao
AU - He, Dongmei
AU - Shen, Xiao
AU - Wang, Lisheng
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China (grant number 81373973 ), Natural Science Foundation of China (grant number 81573872 ), Science Program for Overseas Scholar of Guangzhou University of Chinese Medicine (Torch Program XH20170108 ), Major program for the fundamental research of the education department of Guangdong province (grant number 2017KZDXM018 ) and a Guangdong Provincial Science and Technology projects (grant number 2013B032500015 ). All authors have declared no conflict of interest.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/8/15
Y1 - 2018/8/15
N2 - BuyangHuanwu decoction (BHD) is widely used as a traditional herbal medicine because of its antithrombotic effect, which is attributed to the inhibition of platelet aggregation; however, its active compounds remain unknown. In this study, we developed a method involving platelet binding, solid-phase extraction, and HPLC-MS/MS for screening BHD compounds with potential anti-platelet aggregation properties. Five compounds showing platelet binding affinity were identified as 6-hydroxykaempferol-di-O-glucoside, paeoniflorin, calycosin-7-O-β-D-glucoside, galloylpaeoniflorin, and formononetin-7-O-β-D-glucoside. The results of anti-platelet aggregation experiments in vitro confirmed that these compounds inhibited adenosine diphosphate-induced platelet aggregation. Our results suggest that a platelet binding assay combined with solid-phase extraction and HPLC-MS/MS is an effective method for screening anti-platelet aggregation agents in traditional Chinese medicines.
AB - BuyangHuanwu decoction (BHD) is widely used as a traditional herbal medicine because of its antithrombotic effect, which is attributed to the inhibition of platelet aggregation; however, its active compounds remain unknown. In this study, we developed a method involving platelet binding, solid-phase extraction, and HPLC-MS/MS for screening BHD compounds with potential anti-platelet aggregation properties. Five compounds showing platelet binding affinity were identified as 6-hydroxykaempferol-di-O-glucoside, paeoniflorin, calycosin-7-O-β-D-glucoside, galloylpaeoniflorin, and formononetin-7-O-β-D-glucoside. The results of anti-platelet aggregation experiments in vitro confirmed that these compounds inhibited adenosine diphosphate-induced platelet aggregation. Our results suggest that a platelet binding assay combined with solid-phase extraction and HPLC-MS/MS is an effective method for screening anti-platelet aggregation agents in traditional Chinese medicines.
KW - Anti-platelet aggregation
KW - BuyangHuanwu decoction
KW - Platelet binding assay
KW - Solid phase extraction
KW - Traditional Chinese medicines
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U2 - 10.1016/j.jchromb.2018.06.027
DO - 10.1016/j.jchromb.2018.06.027
M3 - Article
C2 - 29936367
AN - SCOPUS:85048803163
VL - 1092
SP - 320
EP - 327
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
ER -