Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain

J. P. Puma, T. G. Fanning, L. J T Young, Robert Cardiff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We examined the genetic structure, in terms of restriction endonuclease recognition sites, of the milk-transmitted, low-oncogenic mouse mammary tumor virus (MuMTV) of the BALB/cNIV mouse strain. An analysis with EcoRI documented the presence of acquired cNIV proviruses in the mammary tumor DNAs of BALB/cNIV animals. A comparison of tumor DNAs digested with PstI showed that both the cNIV MuMTV and C3Hf MuMTV proviruses lacked the 4.3- and 1.1-kilobase pair fragments characteristic of C3H MuMTV patterns. An examination of mammary tumor and normal, nonmammary tissue DNAs with BamHI supported the idea that the cNIV MuMTV is identical to the C3Hf MuMTV and demonstrated that these two low-oncogenic proviruses are identical to the high-oncogenic C3H MuMTV provirus with respect to a pair of BamHI sites which define a 1.3-kilobase pair fragment. For each of the three MuMTV strains, we also mapped DNAs generated in isolated virions by reverse transcription of their genomic RNAs. Our results showed that cNIV and C3Hf MuMTV are distinct entities by virtue of an additional PstI site within the cNIV long terminal repeat sequence. Another unique feature of cNIV MuMTV revealed by the analysis of virion-generated DNAs was the existence of a family of genomes within the cNIV population. We concluded that cNIV is distinct from its presumptive C3Hf MuMTV predecessor.

Original languageEnglish (US)
Pages (from-to)158-165
Number of pages8
JournalJournal of Virology
Volume43
Issue number1
StatePublished - Jan 1 1982

Fingerprint

Mouse mammary tumor virus
mice
proviruses
Proviruses
DNA
terminal repeat sequences
Terminal Repeat Sequences
Inbred C3H Mouse
mammary neoplasms (animal)
virion
Virion
Breast Neoplasms
reverse transcription
Genetic Structures
DNA Restriction Enzymes
restriction endonucleases
Reverse Transcription

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain. / Puma, J. P.; Fanning, T. G.; Young, L. J T; Cardiff, Robert.

In: Journal of Virology, Vol. 43, No. 1, 01.01.1982, p. 158-165.

Research output: Contribution to journalArticle

Puma, J. P. ; Fanning, T. G. ; Young, L. J T ; Cardiff, Robert. / Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain. In: Journal of Virology. 1982 ; Vol. 43, No. 1. pp. 158-165.
@article{862301b105584722ab96b0b55bf99d45,
title = "Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain",
abstract = "We examined the genetic structure, in terms of restriction endonuclease recognition sites, of the milk-transmitted, low-oncogenic mouse mammary tumor virus (MuMTV) of the BALB/cNIV mouse strain. An analysis with EcoRI documented the presence of acquired cNIV proviruses in the mammary tumor DNAs of BALB/cNIV animals. A comparison of tumor DNAs digested with PstI showed that both the cNIV MuMTV and C3Hf MuMTV proviruses lacked the 4.3- and 1.1-kilobase pair fragments characteristic of C3H MuMTV patterns. An examination of mammary tumor and normal, nonmammary tissue DNAs with BamHI supported the idea that the cNIV MuMTV is identical to the C3Hf MuMTV and demonstrated that these two low-oncogenic proviruses are identical to the high-oncogenic C3H MuMTV provirus with respect to a pair of BamHI sites which define a 1.3-kilobase pair fragment. For each of the three MuMTV strains, we also mapped DNAs generated in isolated virions by reverse transcription of their genomic RNAs. Our results showed that cNIV and C3Hf MuMTV are distinct entities by virtue of an additional PstI site within the cNIV long terminal repeat sequence. Another unique feature of cNIV MuMTV revealed by the analysis of virion-generated DNAs was the existence of a family of genomes within the cNIV population. We concluded that cNIV is distinct from its presumptive C3Hf MuMTV predecessor.",
author = "Puma, {J. P.} and Fanning, {T. G.} and Young, {L. J T} and Robert Cardiff",
year = "1982",
month = "1",
day = "1",
language = "English (US)",
volume = "43",
pages = "158--165",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain

AU - Puma, J. P.

AU - Fanning, T. G.

AU - Young, L. J T

AU - Cardiff, Robert

PY - 1982/1/1

Y1 - 1982/1/1

N2 - We examined the genetic structure, in terms of restriction endonuclease recognition sites, of the milk-transmitted, low-oncogenic mouse mammary tumor virus (MuMTV) of the BALB/cNIV mouse strain. An analysis with EcoRI documented the presence of acquired cNIV proviruses in the mammary tumor DNAs of BALB/cNIV animals. A comparison of tumor DNAs digested with PstI showed that both the cNIV MuMTV and C3Hf MuMTV proviruses lacked the 4.3- and 1.1-kilobase pair fragments characteristic of C3H MuMTV patterns. An examination of mammary tumor and normal, nonmammary tissue DNAs with BamHI supported the idea that the cNIV MuMTV is identical to the C3Hf MuMTV and demonstrated that these two low-oncogenic proviruses are identical to the high-oncogenic C3H MuMTV provirus with respect to a pair of BamHI sites which define a 1.3-kilobase pair fragment. For each of the three MuMTV strains, we also mapped DNAs generated in isolated virions by reverse transcription of their genomic RNAs. Our results showed that cNIV and C3Hf MuMTV are distinct entities by virtue of an additional PstI site within the cNIV long terminal repeat sequence. Another unique feature of cNIV MuMTV revealed by the analysis of virion-generated DNAs was the existence of a family of genomes within the cNIV population. We concluded that cNIV is distinct from its presumptive C3Hf MuMTV predecessor.

AB - We examined the genetic structure, in terms of restriction endonuclease recognition sites, of the milk-transmitted, low-oncogenic mouse mammary tumor virus (MuMTV) of the BALB/cNIV mouse strain. An analysis with EcoRI documented the presence of acquired cNIV proviruses in the mammary tumor DNAs of BALB/cNIV animals. A comparison of tumor DNAs digested with PstI showed that both the cNIV MuMTV and C3Hf MuMTV proviruses lacked the 4.3- and 1.1-kilobase pair fragments characteristic of C3H MuMTV patterns. An examination of mammary tumor and normal, nonmammary tissue DNAs with BamHI supported the idea that the cNIV MuMTV is identical to the C3Hf MuMTV and demonstrated that these two low-oncogenic proviruses are identical to the high-oncogenic C3H MuMTV provirus with respect to a pair of BamHI sites which define a 1.3-kilobase pair fragment. For each of the three MuMTV strains, we also mapped DNAs generated in isolated virions by reverse transcription of their genomic RNAs. Our results showed that cNIV and C3Hf MuMTV are distinct entities by virtue of an additional PstI site within the cNIV long terminal repeat sequence. Another unique feature of cNIV MuMTV revealed by the analysis of virion-generated DNAs was the existence of a family of genomes within the cNIV population. We concluded that cNIV is distinct from its presumptive C3Hf MuMTV predecessor.

UR - http://www.scopus.com/inward/record.url?scp=0019956670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019956670&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 158

EP - 165

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -