Identification of a repressor gene involved in the regulation of NAD de novo biosynthesis in Salmonella typhimurium.

N. Zhu, B. M. Olivera, J. R. Roth

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Mutations at the nadI locus affect expression of the first two genes of NAD synthesis, nadA and nadB, which are unlinked. Genetic data imply that the regulatory effects of nadI mutations are not due to indirect consequences of physiological alterations. Two types of mutations map in the nadI region. Common null mutations (nadI) show constitutive high-level expression of the nadB and nadA genes. Rare nadIs mutations cause constitutive low-level expression of nadB and nadA. Some nadIs mutations shut off the expression of the biosynthetic genes sufficiently to cause a nicotinic acid auxotrophy. Spontaneous revertants of auxotrophic nadIs mutants have a NadI- phenotype, including some with deletions of the nadI locus. The nadI locus encodes a repressor protein acting on the unlinked nadA and nadB genes.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalJournal of Bacteriology
Volume170
Issue number1
StatePublished - Jan 1988
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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