Identification of a region of RyR1 that participates in allosteric coupling with the α1S (Cav1.1) II-III loop

Catherine Proenza, Jennifer O'Brien, Junichi Nakai, Santwana Mukherjee, Paul D. Allen, Kurt G. Beam

Research output: Contribution to journalArticle

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Abstract

In skeletal muscle, excitation-contraction (EC) coupling and retrograde signaling are thought to result from direct interactions between the ryanodine receptor (RyR1) and the α1 subunit of the dihydropyridine receptor (α1S). Previous work has shown that the s53 region of α1S (residues 720-765 in the II-III loop) and regions R10 (1635-2636) and R9 (2659-3720) of RyR1 are involved in this signaling. Using the yeast two-hybrid system, we here report an interaction between s53 and the sR16 region of RyR1 (1837-2168, within R10), whereas no interaction was seen using upstream residues of the α1S II-III loop (s31, 666-709). The specificity of the s53-sR16 interaction was tested by using fragments of the cardiac RyR (RyR2) and DHPR (α1C) that correspond to sR16 and s53, respectively. No interaction was observed for sR16 × c53 (α1C 850-897), but weak interaction was occasionally observed for s53 × cR16 (RyR2 1817-2142). To test the functional significance of the s53 × sR16 interaction, we expressed in dyspedic myotubes a chimeric RyR (chimeraR16) in which sR16 was substituted for the corresponding region of RyR2. ChimeraR16 was found to mediate weak skeletal-type EC coupling. To test the necessity of sR16 sequence for coupling, we used "chimeraR16-rev," in which sR16 and a small upstream region of RyR1 were replaced by RyR2 sequence. ChimeraR16-rev did not differ from RyR1 in its ability to mediate EC coupling. Thus, interaction between residues 720-765 of α1S and residues 1837-2168 of RyR1 appears to contribute to but is not essential for EC coupling in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)6530-6535
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number8
DOIs
StatePublished - Feb 22 2002
Externally publishedYes

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Ryanodine Receptor Calcium Release Channel
Excitation Contraction Coupling
Muscle
Skeletal Muscle
L-Type Calcium Channels
Two-Hybrid System Techniques
Skeletal Muscle Fibers
Hybrid systems
Yeast

ASJC Scopus subject areas

  • Biochemistry

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Identification of a region of RyR1 that participates in allosteric coupling with the α1S (Cav1.1) II-III loop. / Proenza, Catherine; O'Brien, Jennifer; Nakai, Junichi; Mukherjee, Santwana; Allen, Paul D.; Beam, Kurt G.

In: Journal of Biological Chemistry, Vol. 277, No. 8, 22.02.2002, p. 6530-6535.

Research output: Contribution to journalArticle

Proenza, Catherine ; O'Brien, Jennifer ; Nakai, Junichi ; Mukherjee, Santwana ; Allen, Paul D. ; Beam, Kurt G. / Identification of a region of RyR1 that participates in allosteric coupling with the α1S (Cav1.1) II-III loop. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 8. pp. 6530-6535.
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T1 - Identification of a region of RyR1 that participates in allosteric coupling with the α1S (Cav1.1) II-III loop

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AU - O'Brien, Jennifer

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AU - Mukherjee, Santwana

AU - Allen, Paul D.

AU - Beam, Kurt G.

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