Identification of a Novel Papillomavirus in a Northern Fulmar (Fulmarus glacialis) With Viral Production in Cartilage

A. M. Gaynor, S. Fish, R. S. Duerr, F. N Dela Cruz, Patricia Pesavento

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We report the identification of a novel papillomavirus, Fulmarus glacialis papillomavirus 1 (FgPV1), present within an interdigital foot mass of a Northern Fulmar (Fulmarus glacialis). The mass of interest was composed of normal stratified and keratinized epithelium and dense mesenchymal cells with central cartilaginous islands. Within the nuclei of many chondrocytes were loose aggregates or paracrystalline arrays of virions approximately 50 nm in size. Degenerate polymerase chain reaction was used to identify the virus as a putative papillomavirus, and the entire viral genome of 8132 base pairs was subsequently amplified and sequenced. Analysis revealed canonical papillomavirus architecture, including the early open reading frames E6, E7, E1, and E2 and the 2 late proteins L1 and L2. FgPV1 is most closely related to a cluster of avian and reptilian papillomaviruses as visualized by phylogenetic trees. This observation suggests that papillomavirus virion production can occur in mesenchymal cells.

Original languageEnglish (US)
Pages (from-to)553-561
Number of pages9
JournalVeterinary Pathology
Volume52
Issue number3
DOIs
StatePublished - May 3 2015

Fingerprint

Papillomaviridae
cartilage
Virion
Cartilage
Viral Genome
Chondrocytes
Islands
Base Pairing
Open Reading Frames
Foot
Epithelium
Viruses
Polymerase Chain Reaction
virion
Proteins
chondrocytes
cell aggregates
open reading frames
epithelium
polymerase chain reaction

Keywords

  • birds
  • chondrocytes
  • genome—viral
  • histocytochemistry
  • inclusion bodies—viral
  • papillomavirus infections
  • transmission electron microscopy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Identification of a Novel Papillomavirus in a Northern Fulmar (Fulmarus glacialis) With Viral Production in Cartilage. / Gaynor, A. M.; Fish, S.; Duerr, R. S.; Cruz, F. N Dela; Pesavento, Patricia.

In: Veterinary Pathology, Vol. 52, No. 3, 03.05.2015, p. 553-561.

Research output: Contribution to journalArticle

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