Identification of a novel myosin heavy chain gene expressed in the rat larynx

Albert L. Merati, Sue C. Bodine, Thecla Bennett, Hak Hyun Jung, Hiroshi Furuta, Allen F. Ryan

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Based on reactivity to antibodies against known myosin heavy chains, expression of a novel fast myosin heavy chain (MHC) gene was suspected in the thyroarytenoid (TA) muscle of the rat larynx. The 3' ends of MHC transcripts in the TA were amplified by RT-PCR using a primer to a highly conserved MHC sequence and to the poly(A) tail. The resultant products were cloned and fourteen PCR products were screened by dot-blotting with oligonucleotides specific for known skeletal muscle MHC genes. A clone that reacted weakly to the 2B oligo was sequenced and found to encode a novel fast MHC transcript, termed 2L, that appears to represent an eighth vertebrate skeletal muscle MHC gene. By homology analysis, the 2L sequence is most similar to the extraocular MHC, suggesting a possible evolutionary relationship between MHCs associated with the branchial arches.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1306
Issue number2-3
DOIs
StatePublished - May 2 1996

Keywords

  • larynx
  • muscle protein
  • myosin evolution
  • myosin heavy chain gene
  • rat

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

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