Identification of a novel human RAD51 homolog, RAD51B

Joanna S. Albala, Michael P. Thelen, Christa Prange, Fan Wufang, Mari Christensen, Larry H. Thompson, Gregory G. Lennon

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The highly conserved Saccharomyces cerevisiae RAD51 protein functions in both mitotic and meiotic homologous recombination and in double-strand break repair. Screening of the public cDNA sequence database for RAD51-like genes led to the identification of a partial sequence from a breast tissue library present in the I.M.A.G.E. (Integrated Molecular Analysis of Genes and their Expression) collection. An extended 1764-bp cDNA clone encoding an open reading frame of 350 amino acids was isolated. This clone showed significant amino acid identity with other human RAD51 homologs. The new homolog, named RAD51B, was mapped to human chromosome 14q23-q24.2 using a panel of human- hamster somatic cell hybrids and fluorescence in situ bybridization. Northern blot analysis demonstrated that RAD51B mRNA is widely expressed and most abundant in tissues active in recombination. Functions associated with known RAD51 homologs suggest a role for RAD51B in meiotic recombination and/or recombination repair.

Original languageEnglish (US)
Pages (from-to)476-479
Number of pages4
JournalGenomics
Volume46
Issue number3
DOIs
StatePublished - Dec 15 1997
Externally publishedYes

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Genetic Recombination
Rad51 Recombinase
Complementary DNA
Clone Cells
Saccharomyces cerevisiae Proteins
Recombinational DNA Repair
Amino Acids
Hybrid Cells
Homologous Recombination
Human Chromosomes
Cricetinae
Northern Blotting
Open Reading Frames
Breast
Fluorescence
Databases
Gene Expression
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Albala, J. S., Thelen, M. P., Prange, C., Wufang, F., Christensen, M., Thompson, L. H., & Lennon, G. G. (1997). Identification of a novel human RAD51 homolog, RAD51B. Genomics, 46(3), 476-479. https://doi.org/10.1006/geno.1997.5062

Identification of a novel human RAD51 homolog, RAD51B. / Albala, Joanna S.; Thelen, Michael P.; Prange, Christa; Wufang, Fan; Christensen, Mari; Thompson, Larry H.; Lennon, Gregory G.

In: Genomics, Vol. 46, No. 3, 15.12.1997, p. 476-479.

Research output: Contribution to journalArticle

Albala, JS, Thelen, MP, Prange, C, Wufang, F, Christensen, M, Thompson, LH & Lennon, GG 1997, 'Identification of a novel human RAD51 homolog, RAD51B', Genomics, vol. 46, no. 3, pp. 476-479. https://doi.org/10.1006/geno.1997.5062
Albala JS, Thelen MP, Prange C, Wufang F, Christensen M, Thompson LH et al. Identification of a novel human RAD51 homolog, RAD51B. Genomics. 1997 Dec 15;46(3):476-479. https://doi.org/10.1006/geno.1997.5062
Albala, Joanna S. ; Thelen, Michael P. ; Prange, Christa ; Wufang, Fan ; Christensen, Mari ; Thompson, Larry H. ; Lennon, Gregory G. / Identification of a novel human RAD51 homolog, RAD51B. In: Genomics. 1997 ; Vol. 46, No. 3. pp. 476-479.
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