Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae

Viktoria Rungelrath, Jan Christian Wohlsein, Ursula Siebert, Jeffrey L Stott, Ellen Prenger-Berninghoff, Ulrich von Pawel-Rammingen, Peter Valentin-Weigand, Christoph G. Baums, Jana Seele

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Streptococcus (S.) phocae subsp. phocae causes bronchopneumonia and septicemia in a variety of marine mammals. Especially in harbor seals infected with phocine distemper virus it plays an important role as an opportunistic pathogen. This study was initiated by the detection of IgG cleavage products in Western blot analysis after incubation of bacterial supernatant with harbor seal serum. Hence, the objectives of this study were the identification and characterization of a secreted IgG cleaving protease in S. phocae subsp. phocae isolated from marine mammals. To further identify the responsible factor of IgG cleavage a protease inhibitor profile was generated. Inhibition of the IgG cleaving activity by iodoacetamide and Z-LVG-CHN2 indicated that a cysteine protease is involved. Moreover, an anti-IdeS antibody directed against the IgG endopeptidase IdeS of S. pyogenes showed cross reactivity with the putative IgG protease of S. phocae subsp. phocae. The IgG cleaving factor of S. phocae subsp. phocae was identified through an inverse PCR approach and designated IdeP (Immunoglobulin G degrading enzyme of S. phocae subsp. phocae) in analogy to the cysteine protease IdeS. Notably, recombinant (r) IdeP is a host and substrate specific protease as it cleaves IgG from grey and harbor seals but not IgG from harbor porpoises or non-marine mammals. The identification of IdeP represents the first description of a protein in S. phocae subsp. phocae involved in immune evasion. Furthermore, the fact that IdeP cleaves solely IgG of certain marine mammals reflects functional adaption of S. phocae subsp. phocae to grey and harbor seals as its main hosts.

Original languageEnglish (US)
Pages (from-to)42-48
Number of pages7
JournalVeterinary Microbiology
Volume201
DOIs
StatePublished - Mar 1 2017

Fingerprint

Streptococcus phocae
Phoca
Streptococcus
Peptide Hydrolases
proteinases
Immunoglobulin G
Phoca vitulina
marine mammals
cysteine proteinases
Phocine distemper virus
Mammals
immune evasion
Earless Seals
Phocoena phocoena
bronchopneumonia
immunoglobulin G
septicemia
Cysteine Proteases
proteinase inhibitors
cross reaction

Keywords

  • IdeS
  • Immunoglobulin degrading protease
  • Marine mammals
  • Streptococcus phocae

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Rungelrath, V., Wohlsein, J. C., Siebert, U., Stott, J. L., Prenger-Berninghoff, E., von Pawel-Rammingen, U., ... Seele, J. (2017). Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae. Veterinary Microbiology, 201, 42-48. https://doi.org/10.1016/j.vetmic.2017.01.009

Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae. / Rungelrath, Viktoria; Wohlsein, Jan Christian; Siebert, Ursula; Stott, Jeffrey L; Prenger-Berninghoff, Ellen; von Pawel-Rammingen, Ulrich; Valentin-Weigand, Peter; Baums, Christoph G.; Seele, Jana.

In: Veterinary Microbiology, Vol. 201, 01.03.2017, p. 42-48.

Research output: Contribution to journalArticle

Rungelrath, V, Wohlsein, JC, Siebert, U, Stott, JL, Prenger-Berninghoff, E, von Pawel-Rammingen, U, Valentin-Weigand, P, Baums, CG & Seele, J 2017, 'Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae', Veterinary Microbiology, vol. 201, pp. 42-48. https://doi.org/10.1016/j.vetmic.2017.01.009
Rungelrath V, Wohlsein JC, Siebert U, Stott JL, Prenger-Berninghoff E, von Pawel-Rammingen U et al. Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae. Veterinary Microbiology. 2017 Mar 1;201:42-48. https://doi.org/10.1016/j.vetmic.2017.01.009
Rungelrath, Viktoria ; Wohlsein, Jan Christian ; Siebert, Ursula ; Stott, Jeffrey L ; Prenger-Berninghoff, Ellen ; von Pawel-Rammingen, Ulrich ; Valentin-Weigand, Peter ; Baums, Christoph G. ; Seele, Jana. / Identification of a novel host-specific IgG protease in Streptococcus phocae subsp. phocae. In: Veterinary Microbiology. 2017 ; Vol. 201. pp. 42-48.
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abstract = "Streptococcus (S.) phocae subsp. phocae causes bronchopneumonia and septicemia in a variety of marine mammals. Especially in harbor seals infected with phocine distemper virus it plays an important role as an opportunistic pathogen. This study was initiated by the detection of IgG cleavage products in Western blot analysis after incubation of bacterial supernatant with harbor seal serum. Hence, the objectives of this study were the identification and characterization of a secreted IgG cleaving protease in S. phocae subsp. phocae isolated from marine mammals. To further identify the responsible factor of IgG cleavage a protease inhibitor profile was generated. Inhibition of the IgG cleaving activity by iodoacetamide and Z-LVG-CHN2 indicated that a cysteine protease is involved. Moreover, an anti-IdeS antibody directed against the IgG endopeptidase IdeS of S. pyogenes showed cross reactivity with the putative IgG protease of S. phocae subsp. phocae. The IgG cleaving factor of S. phocae subsp. phocae was identified through an inverse PCR approach and designated IdeP (Immunoglobulin G degrading enzyme of S. phocae subsp. phocae) in analogy to the cysteine protease IdeS. Notably, recombinant (r) IdeP is a host and substrate specific protease as it cleaves IgG from grey and harbor seals but not IgG from harbor porpoises or non-marine mammals. The identification of IdeP represents the first description of a protein in S. phocae subsp. phocae involved in immune evasion. Furthermore, the fact that IdeP cleaves solely IgG of certain marine mammals reflects functional adaption of S. phocae subsp. phocae to grey and harbor seals as its main hosts.",
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