Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis

Simon J. Anthony; Judy A. St. Leger; Isamara Navarrete-Macias; Erika Nilson; Maria Sanchez-Leon; Eliza Liang; Tracie Seimon; Komal Jain; William Karesh; Peter Daszak; Thomas Briese; W. Ian Lipkin

doi:10.1371/journal.pone.0068239

Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis

Simon J. Anthony, Judy A. St. Leger, Isamara Navarrete-Macias, Erika Nilson, Maria Sanchez-Leon, Eliza Liang, Tracie Seimon, Komal Jain, William Karesh, Peter Daszak, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

A female short-beaked common dolphin calf was found stranded in San Diego, California in October 2010, presenting with multifocal ulcerative lesions in the trachea and bronchi. Viral particles suggestive of polyomavirus were detected by EM, and subsequently confirmed by PCR and sequencing. Full genome sequencing (Ion Torrent) revealed a circular dsDNA genome of 5,159 bp that was shown to form a distinct lineage within the genus Polyomavirus based on phylogenetic analysis of the early and late transcriptomes. Viral infection and distribution in laryngeal mucosa was characterised using in-situ hybridisation, and apoptosis observed in the virus-infected region. These results demonstrate that polyomaviruses can be associated with respiratory disease in cetaceans, and expand our knowledge of their diversity and clinical significance in marine mammals.

Original language	English (US)
Article number	e68239
Journal	PLoS One
Volume	8
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0068239
State	Published - Jul 10 2013
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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Anthony, S. J., St. Leger, J. A., Navarrete-Macias, I., Nilson, E., Sanchez-Leon, M., Liang, E., Seimon, T., Jain, K., Karesh, W., Daszak, P., Briese, T., & Lipkin, W. I. (2013). Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. PLoS One, 8(7), [e68239]. https://doi.org/10.1371/journal.pone.0068239

Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. / Anthony, Simon J.; St. Leger, Judy A.; Navarrete-Macias, Isamara; Nilson, Erika; Sanchez-Leon, Maria; Liang, Eliza; Seimon, Tracie; Jain, Komal; Karesh, William; Daszak, Peter; Briese, Thomas; Lipkin, W. Ian.

In: PLoS One, Vol. 8, No. 7, e68239, 10.07.2013.

Research output: Contribution to journal › Article

Anthony, Simon J. ; St. Leger, Judy A. ; Navarrete-Macias, Isamara ; Nilson, Erika ; Sanchez-Leon, Maria ; Liang, Eliza ; Seimon, Tracie ; Jain, Komal ; Karesh, William ; Daszak, Peter ; Briese, Thomas ; Lipkin, W. Ian. / Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. In: PLoS One. 2013 ; Vol. 8, No. 7.

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