Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis

Simon J. Anthony, Judy A. St. Leger, Isamara Navarrete-Macias, Erika Nilson, Maria Sanchez-Leon, Eliza Liang, Tracie Seimon, Komal Jain, William Karesh, Peter Daszak, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A female short-beaked common dolphin calf was found stranded in San Diego, California in October 2010, presenting with multifocal ulcerative lesions in the trachea and bronchi. Viral particles suggestive of polyomavirus were detected by EM, and subsequently confirmed by PCR and sequencing. Full genome sequencing (Ion Torrent) revealed a circular dsDNA genome of 5,159 bp that was shown to form a distinct lineage within the genus Polyomavirus based on phylogenetic analysis of the early and late transcriptomes. Viral infection and distribution in laryngeal mucosa was characterised using in-situ hybridisation, and apoptosis observed in the virus-infected region. These results demonstrate that polyomaviruses can be associated with respiratory disease in cetaceans, and expand our knowledge of their diversity and clinical significance in marine mammals.

Original languageEnglish (US)
Article numbere68239
JournalPLoS One
Volume8
Issue number7
DOIs
StatePublished - Jul 10 2013
Externally publishedYes

Fingerprint

Common Dolphins
Polyomavirus
dolphins
Polyomaviridae
Genes
trachea (vertebrates)
Pulmonary diseases
bronchi
Mammals
genome
marine mammals
Laryngeal Mucosa
Viruses
virion
transcriptome
lesions (animal)
Genome
respiratory tract diseases
in situ hybridization
mucosa

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Anthony, S. J., St. Leger, J. A., Navarrete-Macias, I., Nilson, E., Sanchez-Leon, M., Liang, E., ... Lipkin, W. I. (2013). Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. PLoS One, 8(7), [e68239]. https://doi.org/10.1371/journal.pone.0068239

Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. / Anthony, Simon J.; St. Leger, Judy A.; Navarrete-Macias, Isamara; Nilson, Erika; Sanchez-Leon, Maria; Liang, Eliza; Seimon, Tracie; Jain, Komal; Karesh, William; Daszak, Peter; Briese, Thomas; Lipkin, W. Ian.

In: PLoS One, Vol. 8, No. 7, e68239, 10.07.2013.

Research output: Contribution to journalArticle

Anthony, SJ, St. Leger, JA, Navarrete-Macias, I, Nilson, E, Sanchez-Leon, M, Liang, E, Seimon, T, Jain, K, Karesh, W, Daszak, P, Briese, T & Lipkin, WI 2013, 'Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis', PLoS One, vol. 8, no. 7, e68239. https://doi.org/10.1371/journal.pone.0068239
Anthony SJ, St. Leger JA, Navarrete-Macias I, Nilson E, Sanchez-Leon M, Liang E et al. Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. PLoS One. 2013 Jul 10;8(7). e68239. https://doi.org/10.1371/journal.pone.0068239
Anthony, Simon J. ; St. Leger, Judy A. ; Navarrete-Macias, Isamara ; Nilson, Erika ; Sanchez-Leon, Maria ; Liang, Eliza ; Seimon, Tracie ; Jain, Komal ; Karesh, William ; Daszak, Peter ; Briese, Thomas ; Lipkin, W. Ian. / Identification of a Novel Cetacean Polyomavirus from a Common Dolphin (Delphinus delphis) with Tracheobronchitis. In: PLoS One. 2013 ; Vol. 8, No. 7.
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