Identification of a Mutant Gene Controlling Necrotic Cotyledons in Developing Seedlings of Arabidopsis thaliana

N. B. Tomilova, Alexey Tomilov, O. A. Ogarkova, V. A. Tarasov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Genetic and molecular analyses of an Arabidopsis thaliana mutant with necrotic cotyledons from the collection of insertion mutants obtained earlier were conducted. The mutation under study showed incomplete dominance and represented a single insertion of the T region of pLD3 vector used for transformation of germinating seeds to the plant genome during the creation of the collection. Using TAIL-PCR, a fragment of the mutant DNA adjacent to the left border of the T-DNA insertion was isolated and sequenced. Computer r-aided analysis showed that the insertion was located on the left arm of chromosome 1. The open reading frame containing the insertion has one exon and encodes a protein of 446 amino acids, whose function is unknown.

Original languageEnglish (US)
Pages (from-to)392-400
Number of pages9
JournalRussian Journal of Genetics
Volume37
Issue number4
DOIs
StatePublished - Apr 1 2001
Externally publishedYes

Fingerprint

Plant Genome
Cotyledon
Chromosomes, Human, Pair 1
Seedlings
Arabidopsis
Open Reading Frames
Molecular Biology
Exons
Seeds
Amino Acids
Polymerase Chain Reaction
Mutation
DNA
Genes
Proteins
T-DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a Mutant Gene Controlling Necrotic Cotyledons in Developing Seedlings of Arabidopsis thaliana. / Tomilova, N. B.; Tomilov, Alexey; Ogarkova, O. A.; Tarasov, V. A.

In: Russian Journal of Genetics, Vol. 37, No. 4, 01.04.2001, p. 392-400.

Research output: Contribution to journalArticle

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