Identification of a maize neocentromere in an oat-maize addition line

C. N. Topp, R. J. Okagaki, Juliana R Melo, R. G. Kynast, R. L. Phillips, R. K. Dawe

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

We report a neocentromere event on maize chromosome 3 that occurred due to chromosome breakage. The neocentromere lies on a fragment of the short arm that lacks the primary centromere DNA elements, CentC and CRM. It is transmitted in the genomic background of oat via a new centromere (and kinetochore), as shown by immunolocalization of the oat CENH3 protein. Despite normal transmission of the maize fragment in most progeny, neocentromeres appear to vary in size within the same tissue, as shown by fluorescent measurements. A secondary truncation in one line lowered mitotic transmission to 3% and precipitously reduced the size of the chromosome. The results support the view that neocentromere formation is generally associated with major genomic disturbances such as wide species crosses or deletion of an existing centromere. The data further suggest that new centromeres may undergo a period of instability that is corrected over a period of several generations.

Original languageEnglish (US)
Pages (from-to)228-238
Number of pages11
JournalCytogenetic and Genome Research
Volume124
Issue number3-4
DOIs
StatePublished - Jun 2009
Externally publishedYes

Keywords

  • CENH3
  • Centromere
  • Epigenetic
  • Kinetochore
  • Maize
  • Neocentromere
  • Oat

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

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