Identification of a Gene Involved in the Control of the Root System Development in Arabidopsis thaliana

Alexey Tomilov, N. B. Tomilova, O. A. Ogarkova, V. A. Tarasov

Research output: Contribution to journalArticle

Abstract

Genetic and molecular genetic analysis of a lethal root mutant of Arabidopsis thaliana was carried out. The mutant was obtained from a collection created earlier by means of insertion mutagenesis. The mutation was found to be recessive. It was caused by an insertion of the T region of vector pLD3 used for transformation of germinating seeds when creating the collection of insertion mutants. A 118-bp DNA fragment flanking the left border of the insertion was isolated using the TAIL PCR technique, and its nucleotide sequence was determined. Computer analysis of this DNA region demonstrated that it was located in exon 32 of the YUP8H12R.44 gene in chromosome 1.

Original languageEnglish (US)
Pages (from-to)30-38
Number of pages9
JournalRussian Journal of Genetics
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Molecular Computers
Chromosomes, Human, Pair 1
Arabidopsis
Mutagenesis
Molecular Biology
Exons
Seeds
Polymerase Chain Reaction
Mutation
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a Gene Involved in the Control of the Root System Development in Arabidopsis thaliana. / Tomilov, Alexey; Tomilova, N. B.; Ogarkova, O. A.; Tarasov, V. A.

In: Russian Journal of Genetics, Vol. 37, No. 1, 01.01.2001, p. 30-38.

Research output: Contribution to journalArticle

Tomilov, Alexey ; Tomilova, N. B. ; Ogarkova, O. A. ; Tarasov, V. A. / Identification of a Gene Involved in the Control of the Root System Development in Arabidopsis thaliana. In: Russian Journal of Genetics. 2001 ; Vol. 37, No. 1. pp. 30-38.
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