Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats

Timothy A. Chan, Christian A. Chu, Katherine A Rauen, Michael Kroiher, Suzanna M. Tatarewicz, Robert E. Steele

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Using the polymerase chain reaction with primers corresponding to conserved regions in the kinase domain of protein-tyrosine kinases, we amplified segments of several protein-tyrosine kinase genes from Hydra vulgar is, a member of the ancient metazoan phylum Cnidaria. Characterization of cDNA clones for one of these genes, HTK16, revealed that it encodes a nonreceptor protein-tyrosine kinase with two SH2 domains but no SH3 domain. In this regard the predicted HTK16 protein resembles two mammalian non-receptor protein-tyrosine kinases, the products of the ZAP-70 and syk genes. However, the HTK16 protein contains five ankyrin-like repeats, a structural motif which has not previously been found in protein-tyrosine kinases. The HTK16 protein also contains a potential tyrosine phosphorylation site in its carboxyl-terminal tail which resembles the phosphorylation site in members of the src family. RNA hybridization analysis indicates that the HTK16 gene is expressed in epithelial cells, cells which also express the Hydra homologue of the src protein. Our finding of the HTK16 gene in Hydra indicates that diversification of genes encoding non-receptor protein-tyrosine kinases was a very early event in metazoan evolution.

Original languageEnglish (US)
Pages (from-to)1253-1259
Number of pages7
JournalOncogene
Volume9
Issue number4
StatePublished - Apr 1994

Fingerprint

Ankyrin Repeat
src Homology Domains
Protein-Tyrosine Kinases
Hydra
Genes
Proteins
ZAP-70 Protein-Tyrosine Kinase
Phosphorylation
Cnidaria
Tyrosine
Tail
Phosphotransferases
Complementary DNA
Clone Cells
Epithelial Cells
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Chan, T. A., Chu, C. A., Rauen, K. A., Kroiher, M., Tatarewicz, S. M., & Steele, R. E. (1994). Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats. Oncogene, 9(4), 1253-1259.

Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats. / Chan, Timothy A.; Chu, Christian A.; Rauen, Katherine A; Kroiher, Michael; Tatarewicz, Suzanna M.; Steele, Robert E.

In: Oncogene, Vol. 9, No. 4, 04.1994, p. 1253-1259.

Research output: Contribution to journalArticle

Chan, TA, Chu, CA, Rauen, KA, Kroiher, M, Tatarewicz, SM & Steele, RE 1994, 'Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats', Oncogene, vol. 9, no. 4, pp. 1253-1259.
Chan, Timothy A. ; Chu, Christian A. ; Rauen, Katherine A ; Kroiher, Michael ; Tatarewicz, Suzanna M. ; Steele, Robert E. / Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats. In: Oncogene. 1994 ; Vol. 9, No. 4. pp. 1253-1259.
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