Identification of a fourth family of lycopene cyclases in photosynthetic bacteria

Julia A. Maresca, Joel E. Graham, Martin Wu, Jonathan A Eisen, Donald A. Bryant

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A fourth and large family of lycopene cyclases was identified in photosynthetic prokaryotes. The first member of this family, encoded by the cruA gene of the green sulfur bacterium Chlorobium tepidum, was identified in a complementation assay with a lycopene-producing strain of Escherichia coli. Orthologs of cruA are found in all available green sulfur bacterial genomes and in all cyanobacterial genomes that lack genes encoding CrtL- or CrtY-type lycopene cyclases. The cyanobacterium Synechococcus sp. PCC 7002 has two homologs of CruA, denoted CruA and CruP, and both were shown to have lycopene cyclase activity. Although all characterized lycopene cyclases in plants are CrtL-type proteins, genes orthologous to cruP also occur in plant genomes. The CruA- and CruP-type carotenoid cyclases are members of the FixC dehydrogenase superfamily and are distantly related to CrtL- and CrtY-type lycopene cyclases. Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria.

Original languageEnglish (US)
Pages (from-to)11784-11789
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number28
DOIs
StatePublished - Jul 10 2007

Fingerprint

Bacteria
Chlorobi
Cyanobacteria
Carotenoids
Chlorobium
Genes
Synechococcus
Plant Genome
Bacterial Genomes
Plant Proteins
Biosynthetic Pathways
Sulfur
Oxidoreductases
lycopene cyclase-isomerase
Genome
Escherichia coli

Keywords

  • Carotenogenesis
  • Carotenoids
  • Cyanobacteria
  • Green sulfur bacteria
  • Photosynthesis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of a fourth family of lycopene cyclases in photosynthetic bacteria. / Maresca, Julia A.; Graham, Joel E.; Wu, Martin; Eisen, Jonathan A; Bryant, Donald A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 28, 10.07.2007, p. 11784-11789.

Research output: Contribution to journalArticle

Maresca, Julia A. ; Graham, Joel E. ; Wu, Martin ; Eisen, Jonathan A ; Bryant, Donald A. / Identification of a fourth family of lycopene cyclases in photosynthetic bacteria. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 28. pp. 11784-11789.
@article{6c28cd8741b14443835f9f59ce2681b3,
title = "Identification of a fourth family of lycopene cyclases in photosynthetic bacteria",
abstract = "A fourth and large family of lycopene cyclases was identified in photosynthetic prokaryotes. The first member of this family, encoded by the cruA gene of the green sulfur bacterium Chlorobium tepidum, was identified in a complementation assay with a lycopene-producing strain of Escherichia coli. Orthologs of cruA are found in all available green sulfur bacterial genomes and in all cyanobacterial genomes that lack genes encoding CrtL- or CrtY-type lycopene cyclases. The cyanobacterium Synechococcus sp. PCC 7002 has two homologs of CruA, denoted CruA and CruP, and both were shown to have lycopene cyclase activity. Although all characterized lycopene cyclases in plants are CrtL-type proteins, genes orthologous to cruP also occur in plant genomes. The CruA- and CruP-type carotenoid cyclases are members of the FixC dehydrogenase superfamily and are distantly related to CrtL- and CrtY-type lycopene cyclases. Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria.",
keywords = "Carotenogenesis, Carotenoids, Cyanobacteria, Green sulfur bacteria, Photosynthesis",
author = "Maresca, {Julia A.} and Graham, {Joel E.} and Martin Wu and Eisen, {Jonathan A} and Bryant, {Donald A.}",
year = "2007",
month = "7",
day = "10",
doi = "10.1073/pnas.0702984104",
language = "English (US)",
volume = "104",
pages = "11784--11789",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "28",

}

TY - JOUR

T1 - Identification of a fourth family of lycopene cyclases in photosynthetic bacteria

AU - Maresca, Julia A.

AU - Graham, Joel E.

AU - Wu, Martin

AU - Eisen, Jonathan A

AU - Bryant, Donald A.

PY - 2007/7/10

Y1 - 2007/7/10

N2 - A fourth and large family of lycopene cyclases was identified in photosynthetic prokaryotes. The first member of this family, encoded by the cruA gene of the green sulfur bacterium Chlorobium tepidum, was identified in a complementation assay with a lycopene-producing strain of Escherichia coli. Orthologs of cruA are found in all available green sulfur bacterial genomes and in all cyanobacterial genomes that lack genes encoding CrtL- or CrtY-type lycopene cyclases. The cyanobacterium Synechococcus sp. PCC 7002 has two homologs of CruA, denoted CruA and CruP, and both were shown to have lycopene cyclase activity. Although all characterized lycopene cyclases in plants are CrtL-type proteins, genes orthologous to cruP also occur in plant genomes. The CruA- and CruP-type carotenoid cyclases are members of the FixC dehydrogenase superfamily and are distantly related to CrtL- and CrtY-type lycopene cyclases. Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria.

AB - A fourth and large family of lycopene cyclases was identified in photosynthetic prokaryotes. The first member of this family, encoded by the cruA gene of the green sulfur bacterium Chlorobium tepidum, was identified in a complementation assay with a lycopene-producing strain of Escherichia coli. Orthologs of cruA are found in all available green sulfur bacterial genomes and in all cyanobacterial genomes that lack genes encoding CrtL- or CrtY-type lycopene cyclases. The cyanobacterium Synechococcus sp. PCC 7002 has two homologs of CruA, denoted CruA and CruP, and both were shown to have lycopene cyclase activity. Although all characterized lycopene cyclases in plants are CrtL-type proteins, genes orthologous to cruP also occur in plant genomes. The CruA- and CruP-type carotenoid cyclases are members of the FixC dehydrogenase superfamily and are distantly related to CrtL- and CrtY-type lycopene cyclases. Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria.

KW - Carotenogenesis

KW - Carotenoids

KW - Cyanobacteria

KW - Green sulfur bacteria

KW - Photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=34547442344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547442344&partnerID=8YFLogxK

U2 - 10.1073/pnas.0702984104

DO - 10.1073/pnas.0702984104

M3 - Article

VL - 104

SP - 11784

EP - 11789

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 28

ER -