Identification of a cytoskeleton-associated glycoprotein from isolated microvilli of a mammary ascites tumor

Coralie A. Carothers Carraway, Goeh Jung, Julie R. Craik, Robert W. Rubin, Kermit L. Carraway

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Microvilli isolated from MAT-C1 13762 ascites tumor cells were extracted with Triton X-100 in phosphate-buffered saline (PBS) to yield cytoskeletal residues. Analysis of the residues by two-dimensional isoelectric focusing-dodecyl sulfate electrophoresis and silver staining suggested that one of the major components is a glycoprotein (CAG). Neuraminidase treatments and glucosamine labeling demonstrated that CAG is a glycoprotein, and lactoperoxidase iodination showed its presence at the microvillar surface. DNase treatments and myosin affinity analysis suggested an association between CAG and the microvillar microfilaments. Thus, CAG has the properties expected of a transmembrane-linking molecule connecting the cell surface to the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalExperimental Cell Research
Volume143
Issue number2
DOIs
StatePublished - Feb 1983
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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