Identification of a cytoplasmic domain important in the polarized expression and clustering of the Kv2.1 K+ channel

Robert H. Scannevin, Hideyuki Murakoshi, Kenneth J. Rhodes, James Trimmer

Research output: Contribution to journalArticle

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Abstract

The voltage-sensitive K+ channel Kv2.1 has a polarized and clustered distribution in neurons. To investigate the basis for this localization, we expressed wild-type Kv2.1 and two COOH-terminal truncation mutants, ΔC318 and ΔC187, in polarized epithelial MDCK cells. These functional channel proteins had differing subcellular localization, in that while both wild- type Kv2.1 and ΔC187 localized to the lateral membrane in high density clusters, ΔC318 was expressed uniformly on both apical and lateral membranes. A chimeric protein containing the hemagglutin in protein from influenza virus and the region of Kv2.1 that differentiates the two truncation mutants (amino acids 536-666) was also expressed in MDCK cells, where it was found in high density clusters similar to those observed for Kv2.1. Polarized expression and clustering of Kv2.1 correlates with detergent solubility, suggesting that interaction with the detergent insoluble cytoskeleton may be necessary for proper localization of this channel.

Original languageEnglish (US)
Pages (from-to)1619-1632
Number of pages14
JournalJournal of Cell Biology
Volume135
Issue number6
DOIs
StatePublished - 1996
Externally publishedYes

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Cluster Analysis
Madin Darby Canine Kidney Cells
Detergents
Proteins
Membranes
Orthomyxoviridae
Cytoskeleton
Solubility
Epithelial Cells
Neurons
Amino Acids

ASJC Scopus subject areas

  • Cell Biology

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Identification of a cytoplasmic domain important in the polarized expression and clustering of the Kv2.1 K+ channel. / Scannevin, Robert H.; Murakoshi, Hideyuki; Rhodes, Kenneth J.; Trimmer, James.

In: Journal of Cell Biology, Vol. 135, No. 6, 1996, p. 1619-1632.

Research output: Contribution to journalArticle

Scannevin, Robert H. ; Murakoshi, Hideyuki ; Rhodes, Kenneth J. ; Trimmer, James. / Identification of a cytoplasmic domain important in the polarized expression and clustering of the Kv2.1 K+ channel. In: Journal of Cell Biology. 1996 ; Vol. 135, No. 6. pp. 1619-1632.
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